Anti-NDST1 Rabbit Polyclonal Antibody for WB, ELISA - P52848

Core Product Specifications and Parameters

Biological Background: NDST1 Function and Localization

• NDST1 is the essential bifunctional enzyme that initiates heparan sulfate modification by sequentially catalyzing N-deacetylation and N-sulfation of glucosamine residues, producing the N-sulfated heparosan required for downstream heparin and heparan sulfate biosynthesis. Related references: PMID:35137078, PMID:9230113, PMID:9744796
• This enzymatic step is rate-limiting and dictates the final sulfation pattern of heparan sulfate, which in turn determines its capacity to interact with proteins such as L-selectin, thereby participating in inflammatory cell adhesion and trafficking.
• NDST1 activity is necessary for the exosomal secretion of syndecan, SDCBP (syntenin), and CD63, highlighting a non-canonical function in the biogenesis or release of extracellular vesicles. Related references: PMID:22660413
• An alternatively spliced isoform (isoform 2) lacks both catalytic activities and functions as a dominant-negative regulator, potentially by altering the stoichiometry of the biosynthetic enzyme complexes.
• Subcellularly, NDST1 is an integral membrane protein of the Golgi apparatus, with dual localization to the cis-Golgi and trans-Golgi network membranes.
• The protein is widely expressed across tissues, but is particularly abundant in heart, liver, and pancreas, consistent with organ systems that require extensive heparan sulfate biosynthesis.
• NDST1 is subject to glycosylation and disulfide bond formation; mutations in the NDST1 gene are associated with intellectual disability, underscoring its critical role in development.

Experimental Guidance and Technical Tips

• The immunogen (aa 40–170) encompasses the N-terminal region shared by both isoform 1 and the dominant-negative isoform 2; thus, the antibody may recognize both splice variants and should be validated in the relevant sample system.
• In Western blotting, the mature NDST1 protein runs at an apparent molecular weight of ~101 kDa; band shifts or multiple bands may arise from glycosylation or disulfide-bonded complexes, so using reducing conditions and appropriate positive controls is advisable.
• For ELISA, this polyclonal antibody can serve as a capture or detection reagent; end-users should perform checkerboard titrations with their specific target antigen (recombinant protein or cell lysate) to optimize performance.
• As cross-reactivity to mouse NDST1 is reported, the antibody is suitable for mouse studies; nonetheless, confirm epitope conservation by sequence alignment and pilot experiments in mouse-derived samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories by providing one-stop access to high-quality IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. Whether you are developing assays focused on heparan sulfate metabolism, inflammation, or extracellular vesicle biology, our NDST1 Rabbit pAb serves as a reliable reagent for WB and ELISA applications. Through our expert technical consulting and rare-target sourcing capabilities, CamelBio stands ready to accelerate your project from concept to clinic.