Anti-NDRG2 Rabbit Polyclonal Antibody for WB, ELISA - Q9UN36

Core Product Specifications and Parameters

Biological Background: NDRG2 Function and Localization

• Protein NDRG2, also designated N-myc downstream-regulated gene 2 protein or Syld709613, is implicated in tumor suppression and developmental processes.
• It contributes to the regulation of the Wnt signaling pathway by down-regulating CTNNB1-mediated transcriptional activation of target genes such as CCND1, suggesting a tumor suppressor role.
• NDRG2 may also be involved in dendritic cell differentiation and neuron differentiation.
• Subcellularly, the protein localizes to the cytoplasm, with enrichment in the perinuclear region, and is found in cell projection growth cones.
• Tissue expression is highest in brain, heart, skeletal muscle, and salivary gland, with moderate levels in kidney and liver. It is expressed in dendritic cells but not in other blood cells. Expression is low or absent in several cancer types, including pancreatic, liver, and glioblastoma.
• Two major isoforms (isoform 1 and isoform 2) are present in brain neurons and are up-regulated in Alzheimer disease.
• Post-translational modifications include acetylation and phosphorylation, and the protein undergoes alternative splicing, highlighting regulatory complexity.

Experimental Guidance and Technical Tips

• For Western blotting, consider titrating the antibody to an optimal working concentration. Validate reactivity using positive control lysates from human or mouse tissues with known NDRG2 expression, such as brain or heart.
• In ELISA applications, the recombinant immunogen (amino acids 138-357) can serve as a useful standard for assay development.
• Note the predicted molecular weight of NDRG2 is approximately 41 kDa; however, PTMs may cause slight shifts.
• Upon receipt, store aliquots at -20°C and avoid repeated freeze-thaw cycles to maintain stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research labs to streamline IVD raw-material procurement. For NDRG2-related cancer and neurobiology projects, we can supply validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and assist in sourcing rare target raw materials. Our integrated services support you from concept to clinic, ensuring reliable supply chains for your assay development.