Anti-NDRG1 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q92597

Core Product Specifications and Parameters

Biological Background: NDRG1 Function and Localization

• NDRG1 (N-myc downstream-regulated gene 1) is a stress-responsive protein also known as DRG-1, Cap43, RTP, and Rit42, implicated in hormone responses, cell growth, and differentiation.
• It functions as a tumor suppressor in many cell types and is necessary (but not sufficient) for p53/TP53-mediated caspase activation and apoptosis.
• NDRG1 plays a critical role in cell trafficking, particularly in Schwann cells, and is essential for the maintenance and development of the peripheral nerve myelin sheath; it is also required for vesicular recycling of CDH1 and TF and may participate in lipid trafficking.
• The protein localizes to multiple subcellular compartments, including the cytoplasm, cytosol, centrosome, nucleus, and cell membrane, reflecting its diverse functional roles.
• It is phosphorylated and acetylated, and these post-translational modifications likely regulate its activity in microtubule dynamics and mitotic spindle checkpoint control, thereby protecting cells from spindle disruption damage and maintaining euploidy.
• NDRG1 is ubiquitously expressed, with highest levels in placental membranes, prostate, kidney, small intestine, and ovary; it is mainly found in epithelial cells and Schwann cells, and its expression is often reduced in adenocarcinomas compared to normal tissues.
• This protein is associated with Charcot-Marie-Tooth disease type 4D (CMT4D) and other neuropathies, underlining its importance in peripheral nerve function.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 295-394 of human NDRG1; this region may be conserved across species, supporting detection in human, mouse, and rat samples.
• For Western blot (WB), the predicted molecular weight is approximately 43 kDa; consider using appropriate controls and optimizing antibody concentration to minimize background.
• In immunohistochemistry (paraffin-embedded sections, IHC-P), antigen retrieval may be necessary; validate in relevant tissue types known to express NDRG1, such as kidney or prostate.
• For ELISA applications, the antibody can be used as a capture or detection reagent; pairing with a different clone or polyclonal antibody may improve sensitivity; perform checkerboard titrations to determine optimal working concentrations.
• Always include positive and negative controls in each experiment, and consider the specific cellular context, as NDRG1 expression is downregulated in many tumors.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw material procurement, offering validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents to diagnostic manufacturers and research laboratories worldwide. With NDRG1's involvement in tumor suppression and neuropathy, this antibody supports targeted research in oncology and neurobiology; our team can assist with sourcing complementary capture and detection reagents for ELISA development or rare target raw materials. Whether you need established catalog products or custom development, CamelBio bridges your concept to clinic with reliable, one-stop supply solutions.