Anti-NDP52 Rabbit Monoclonal Antibody for WB, ELISA - Q13137

Core Product Specifications and Parameters

Biological Background: Calcium-binding and coiled-coil domain-containing protein 2 Function and Localization

• Calcium-binding and coiled-coil domain-containing protein 2 (CALCOCO2), also known as NDP52 (Nuclear domain 10 protein 52), Nuclear domain 10 protein NDP52, Nuclear dot protein 52, or Antigen nuclear dot 52 kDa protein.
• Serves as a xenophagy-specific receptor that restricts intracellular bacterial proliferation by targeting pathogens like Salmonella typhimurium for autophagic degradation upon galectin-sensed membrane damage (PubMed:22246324).
  Related references:
  PMID:22246324
• Coordinates the targeting of bacteria to autophagosomes through interactions with MAP1LC3A, MAP1LC3B, and/or GABARAPL2, and subsequently regulates pathogen-containing autophagosome maturation via interaction with MAP3LC3C (PubMed:23022382, PubMed:25771791).
  Related references:
  PMID:23022382
  PMID:25771791
• Localizes to the cytoplasm with enrichment in the perinuclear region, associates with the cytoskeleton, and is recruited to the autophagosome membrane during xenophagy.
• Expressed in all tissues tested, with highest levels in skeletal muscle and lowest in brain.
• May modulate ruffle formation and actin cytoskeleton organization, and negatively regulate constitutive secretion (PubMed:17635994).
  Related references:
  PMID:17635994
• Contains calcium-binding domains, coiled-coil regions, a zinc finger (ZnF) domain, and undergoes phosphorylation; classified under the CALCOCO family with roles in autophagy and host-pathogen interactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-283) of human NDP52; consider this epitope location when designing blocking peptides or epitope mapping experiments.
• Validated for Western blotting and ELISA; optimize antibody dilution and incubation conditions according to your specific assay system.
• Reactivity has been confirmed for human NDP52; if cross-reactivity with orthologs from other species is required, validate experimentally in the relevant sample.
• For autophagy assays, combine with LC3 or other autophagosome markers, and use appropriate controls (e.g., uninfected vs. Salmonella-infected cells) to confirm xenophagy-specific responses.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents, to support diagnostic and research applications. For autophagy and infectious disease studies, we can source rare-target antibodies like this anti-NDP52 antibody, along with technical consulting services to help accelerate your assay development from concept to clinic.