Anti-NDEL1 (NUDEL) Rabbit Polyclonal Antibody for WB, ELISA - Q9GZM8

Core Product Specifications and Parameters

Biological Background: NDEL1 (NUDEL) Function and Localization

• Encoded by the NDEL1 gene, this protein is also known as Nuclear distribution protein nudE-like 1 (NUDEL) or Mitosin-associated protein 1 (MITAP1). It is a key regulator of microtubule organization and dynein-mediated processes.
• Required for organizing the cellular microtubule array and anchoring microtubules at the centrosome, partly by targeting the microtubule-severing protein KATNA1 to the centrosome.
• Positively regulates dynein motor activity by targeting dynein to microtubule plus ends, enabling dynein-dependent functions such as maintenance of Golgi integrity, centripetal motion of secretory vesicles, and coupling of nucleus and centrosome.
• Essential for neuronal migration during brain development: guides newly formed neurons from the ventricular/subventricular zone to the cortical plate, and together with DISC1, regulates neurite outgrowth.
• May act as a RAB9A/B effector that tethers RAB9-associated late endosomes to the dynein motor for retrograde transport to the trans-Golgi network. Related references: PMID:34793709
• Facilitates polymerization of neurofilaments from NEFH and NEFL subunits and positively regulates lysosome peripheral distribution in osteoclasts (by similarity).
• Subcellularly localizes to the cytoplasm, cytoskeleton, centrosome, kinetochore, and mitotic spindle. Widely expressed in brain, heart, kidney, liver, lung, pancreas, placenta, and skeletal muscle. Features a coiled-coil domain and is subject to palmitoylation and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-345 of human NDEL1 (NP_110435.1), covering nearly the full-length protein. This broad immunogen design may contribute to reliable detection in both denaturing western blot (WB) and native ELISA applications.
• For western blotting, the predicted molecular weight is approximately 38 kDa; consider loading appropriate protein ladders and validating band size in relevant human or mouse cell/tissue lysates.
• Given reported human and mouse cross-reactivity, this antibody is suitable for comparative studies across these species. We recommend initial titration and optimization of antibody dilution for each application and sample type.
• For ELISA, consider using recombinant NDEL1 protein as a standard for quantitative assays, and validate performance with your own experimental system.

CamelBio: Your One-Stop Sourcing Bridge

As your dedicated IVD raw-material partner, CamelBio provides comprehensive access to high-quality antibodies, recombinant proteins, and ancillary reagents for microtubule and neurobiology research. Whether you need validated antibody pairs, bulk monoclonals/polyclonals, or rare target sourcing, we streamline your procurement from concept to clinic. Contact us to discuss tailored solutions for your diagnostic development needs.