Anti-NDE1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9NXR1

Core Product Specifications and Parameters

Biological Background: NDE1 Function and Localization

• Nuclear distribution protein nudE homolog 1 (NDE1, also known as NUDEL) is a key regulator of cell division and neurogenesis. It belongs to the nudE family of microtubule-associated proteins.
• NDE1 is essential for centrosome duplication and proper mitotic spindle formation, ensuring accurate chromosome segregation during mitosis.
• In the developing cerebral cortex, NDE1 controls the orientation of the mitotic spindle in neuronal progenitors; a perpendicular division yields two progenitors, while a parallel division produces one progenitor and one neuron, thus regulating the balance between proliferation and differentiation.
• As a RAB9A/B effector, NDE1 tethers RAB9-associated late endosomes to the dynein motor complex, facilitating their retrograde transport to the trans-Golgi network.
  Related references:
  PMID:34793709
• Subcellularly, NDE1 localizes to the cytoplasm, centrosome, spindle apparatus, kinetochore, cleavage furrow, and cytoplasmic vesicle membranes, consistent with its roles in mitosis and endosomal trafficking.
• NDE1 is expressed primarily in the neuroepithelium of the developing brain, including the cerebral cortex and cerebellum, highlighting its importance in cortical development.
• The protein is subject to palmitoylation and phosphorylation, and multiple isoforms exist via alternative splicing; these modifications and variants may modulate its function and localization.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein spanning amino acids 1–110 of human NDE1, which covers the N-terminal region; this region is conserved across human, mouse, and rat, consistent with the predicted cross-reactivity.
• For Western blot (WB), a band around 38 kDa (matching the predicted molecular weight) is expected. We recommend titrating the antibody to determine the optimal dilution for your specific sample and detection system.
• In immunohistochemistry (IHC-P), antigen retrieval and blocking steps should be optimized to achieve strong, specific staining. Use of appropriate tissue controls (e.g., brain or cortex sections) is recommended.
• For ELISA, the polyclonal nature may provide high sensitivity; consider using the antibody in a capture or detection format and confirm specificity with appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, laboratories, and research institutes with a comprehensive portfolio of IVD raw materials. For NDE1-related neurodevelopmental or cell-cycle research, we can supply validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. Our sourcing capabilities also extend to rare target raw materials, helping you bridge from concept to clinic efficiently.