Anti-NDC1 Rabbit Polyclonal Antibody for WB, ELISA - Q9BTX1

Core Product Specifications and Parameters

Biological Background: NDC1 Function and Localization

• NDC1 (Nucleoporin NDC1), also known as NET3 or TMEM48, is a component of the nuclear pore complex (NPC) that mediates nucleocytoplasmic transport.
• It plays a critical role in de novo NPC assembly and insertion into the nuclear envelope, likely by linking the nuclear membrane with soluble nucleoporins to anchor the NPC.
• According to UniProt, NDC1 is localized to the nucleus, specifically the nuclear pore complex and nuclear membrane.
• The protein is a multi-pass transmembrane protein, with transmembrane helices anchoring it in the nuclear envelope.
• NDC1 is implicated in mRNA transport and protein translocation, and mutations are associated with intellectual disability and neuropathy.
• It undergoes alternative splicing and is regulated by phosphorylation, as indicated by proteomics identification.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 340-530 of human NDC1, covering a region likely exposed for antibody recognition; consider this when validating in native and denaturing conditions.
• For WB, a band at ~76kDa is expected in human, mouse, and rat samples; use appropriate loading controls and consider post-translational modifications that may cause shifts.
• In ELISA, titrate the antibody and validate with recombinant NDC1 or peptide antigens to confirm specificity.
• Due to the transmembrane nature of NDC1, include membrane protein extraction protocols to enhance detection sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. For NDC1 and nucleoporin-related diagnostic development, CamelBio is your trusted procurement partner.