Anti-NCTR2 Rabbit Monoclonal Antibody for WB, ELISA - O95944

Core Product Specifications and Parameters

Biological Background: NCTR2 Function and Localization

• NCTR2, also known as NKp44, CD336, or LY95, is a natural cytotoxicity triggering receptor encoded by the NCR2 gene.
• It functions as a cytotoxicity-activating receptor that enhances the ability of activated natural killer (NK) cells to lyse tumor cells.
• The protein is a single-pass type I membrane protein located on the cell membrane (UniProt Keywords: Cell membrane, Transmembrane, Transmembrane helix).
• Selectively expressed on activated NK cells and in vitro cultured TCRγδ lymphoid cells, underlining its role in immune surveillance.
• Contains an immunoglobulin domain, a signal peptide, and disulfide bonds (UniProt Keywords: Immunoglobulin domain, Signal, Disulfide bond, Glycoprotein).
• Undergoes alternative splicing (UniProt Keywords: Alternative splicing) and is subject to post-translational modifications including glycosylation and disulfide bond formation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminal region (aa 1-100) of human NCTR2, which includes the signal peptide and part of the extracellular domain. This design may favor detection of the mature protein.
• For Western blot, consider that NCTR2 has a predicted molecular weight of ~31 kDa (UniProt: 30677 Da), but glycosylation and signal peptide cleavage may result in a band around 31 kDa; validate with appropriate controls.
• For ELISA, the antibody may be used for direct detection or as a capture antibody; consider pairing with a different clone for sandwich ELISA.
• Since expression is restricted to activated NK cells and TCRγδ cells, use appropriate cell lysates or stimulated samples to confirm reactivity in WB.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with streamlined access to validated IVD raw materials. This anti-NCTR2 rabbit monoclonal antibody supports studies in natural killer cell biology and tumor immunity. Complement your project with our range of ancillary reagents, bulk ordering options, and expert technical consulting.