Anti-NCSTN Rabbit pAb for WB, IF/ICC, ELISA - Q92542

Core Product Specifications and Parameters

Biological Background: NCSTN Function and Localization

• As a core component of the gamma-secretase complex, nicastrin facilitates the intramembrane cleavage of integral membrane proteins, notably Notch receptors and amyloid precursor protein (APP).
  Related references: PMID:10993067, PMID:12679784, PMID:25043039
• It plays a critical role in Notch and Wnt signaling pathways by regulating the processing of key regulatory proteins and cytosolic CTNNB1 levels.
• NCSTN is widely expressed, with significant protein-level detection in the brain, underscoring its neurological importance.
  Related references: PMID:10993067, PMID:11396676
• The protein localizes to the plasma membrane, cytoplasmic vesicle membranes, and melanosomes.
• Post-translational modifications include glycosylation and disulfide bond formation, which are crucial for protein stability and function.
• Alternative splicing generates multiple isoforms, potentially contributing to functional diversity.
• Disease variant associations highlight its clinical relevance, particularly in neurodegeneration.

Experimental Guidance and Technical Tips

• The immunogen spans the C-terminal region (aa 429-669) of human nicastrin, targeting a domain likely conserved across human, mouse, and rat, consistent with reported cross-reactivity.
• For Western blot, the expected molecular weight is approximately 78 kDa; consider using reducing conditions and validate in lysates from human, mouse, or rat cell lines.
• In immunofluorescence/immunocytochemistry, anticipate membrane and vesicular staining; optimize permeabilization and fixation protocols for your cell type.
• For ELISA, test both direct and sandwich formats, and pair with a validated capture or detection antibody if needed; optimize coating antigen and antibody concentrations.
• Note that nicastrin undergoes glycosylation; treatment with PNGase F may shift the molecular weight if confirmation is needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw-material procurement, supplying validated antibodies, reagents, and technical support to accelerate research in neurodegenerative diseases and cell signaling. This NCSTN antibody is part of our expanding catalog for gamma-secretase and Alzheimer's biomarker studies. Contact us for bulk orders, customized antibody pairs, or assistance with rare-target reagent sourcing.