Anti-NCS1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P62166

Core Product Specifications and Parameters

Biological Background: NCS1 Function and Localization

• NCS1 (Neuronal calcium sensor 1), also known as frequenin homolog (FLUP, FREQ), is a member of the neuronal calcium sensor family, acting as a calcium-dependent regulator of G protein-coupled receptor phosphorylation.
• It directly regulates rhodopsin kinase GRK1 (RHOK) but not other GRK family members (GRK2 to GRK5) and can substitute for calmodulin in certain contexts.
• NCS1 stimulates phosphatidylinositol 4-kinase beta (PI4KB) kinase activity and is implicated in long-term synaptic plasticity via interaction with PICK1.
• It may play a role in neuron differentiation by inhibiting N-type voltage-gated calcium channel activity.
• The protein is localized to the Golgi apparatus, postsynaptic density, perinuclear cytoplasm, and cell membrane.
• Keywords highlight its involvement in calcium binding, membrane association (myristoylation), alternative splicing, and presence in synapse.
• The predicted molecular weight is approximately 22 kDa.

Experimental Guidance and Technical Tips

• For Western blotting, a band near 22 kDa is expected; consider using positive controls such as brain tissue lysates from human, mouse, or rat.
• In immunocytochemistry/immunofluorescence, permeabilization may be required to detect Golgi or cytoplasmic pools; fixation methods should be optimized for membrane-bound targets.
• For ELISA applications, ensure recombinant protein coating or capture antibodies are validated in your specific buffer systems.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material partner offering a comprehensive portfolio from validated antibody pairs and optimized polyclonal antibodies to bulk ancillary reagents. For researchers investigating neuronal calcium signaling and synaptic plasticity, we provide reliable sourcing of NCS1-targeted antibodies and supportive raw materials. Let CamelBio streamline your procurement, whether you need off-the-shelf reagents or custom supply solutions for rare targets.