Anti-NCS1 Rabbit Polyclonal Antibody for WB, ELISA - P62166

Core Product Specifications and Parameters

Biological Background: NCS1 Function and Localization

• NCS1, also known as Frequenin homolog, Frequenin-like protein, or FLUP, is a neuronal calcium sensor protein belonging to the recoverin family of calcium-binding proteins.
• It functions as a calcium-dependent regulator of G protein-coupled receptor (GPCR) phosphorylation, specifically regulating GRK1 but not GRK2–GRK5, and can substitute for calmodulin.
• NCS1 stimulates phosphatidylinositol 4-kinase beta (PI4KB) activity, linking calcium signaling to phosphoinositide metabolism.
• It is involved in long-term synaptic plasticity through interaction with PICK1, implicating it in learning and memory processes.
• NCS1 may also regulate neuron differentiation by inhibiting N-type voltage-gated calcium channels.
• Subcellularly, NCS1 localizes to the Golgi apparatus, postsynaptic density, perinuclear cytoplasm, cell membrane, and other cytoplasmic regions, reflecting its multiple functional roles.
• The protein contains EF-hand calcium-binding domains and is myristoylated, which facilitates membrane association; it exhibits alternative splicing and has a molecular weight of approximately 22 kDa (predicted 21,879 Da).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-190 of human NCS1, encompassing the full-length protein; consider that this polyclonal antibody may recognize both native and denatured conformations.
• For Western blotting, the predicted molecular weight is approximately 22 kDa; optimize gel percentage (e.g., 12-15% SDS-PAGE) and use appropriate loading controls.
• For ELISA, consider using the recombinant protein as a coating antigen or standard for antibody titration.
• Cross-reactivity with human, mouse, and rat has been specified; validate antibody specificity in the relevant species and tissue types.
• Since NCS1 is a calcium-binding protein, consider the presence of calcium or chelators during sample preparation, as conformational changes may affect epitope recognition.

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