Anti-NCR3 Rabbit Monoclonal Antibody for WB, IF/ICC, FC, ELISA - O14931

Core Product Specifications and Parameters

Biological Background: NCR3 Function and Localization

• NCR3, also designated NKp30 or CD337, is a member of the natural cytotoxicity receptor family and belongs to the immunoglobulin superfamily.
• It functions as an activating receptor selectively expressed on natural killer (NK) cells, where it recognizes stress-induced ligands BAG6 and NCR3LG1 on target cells.
• Ligand binding triggers NK cell cytotoxicity, playing a pivotal role in the immune surveillance against tumor cells.
• Beyond direct cytotoxicity, engagement of NCR3 by BAG6 promotes myeloid dendritic cell maturation through the release of TNFα and IFNγ by NK cells.
• The protein is localized to the cell membrane and features an extracellular immunoglobulin domain, a single transmembrane helix, and a short cytoplasmic tail.
• Post-translational modifications include disulfide bond formation and glycosylation, which may influence its molecular weight and functional properties.
• Alternative splicing generates multiple isoforms, contributing to the observed molecular weight range (16–21 kDa) in experimental settings.
• Tissue expression is largely restricted to resting and activated NK cells, with weak expression detected in the spleen.

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular domain (amino acids 19–135), suggesting the antibody may recognize an epitope accessible in live cell applications; however, it is validated for intracellular flow cytometry—optimization for surface staining may be considered.
• Due to alternative splicing and glycosylation, expect multiple bands or bands between 16 and 21 kDa in WB; validate specificity using positive controls (e.g., NK cell lysates) and include appropriate blocking steps.
• For immunofluorescence and flow cytometry, inclusion of permeabilization and fixation steps is recommended in line with the validated intracellular staining protocol.

CamelBio: Your One-Stop Sourcing Bridge

For researchers and diagnostic developers working on NK cell biology and innate immunity, CamelBio streamlines access to critical raw materials such as Anti-NCR3 antibodies. Our portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, and a wide range of ancillary reagents, supporting your IVD assay development from early-stage research to commercial production. Leverage our expertise to source even rare-target biomaterials efficiently under one roof.