Anti-NCOR2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9Y618

Core Product Specifications and Parameters

Biological Background: NCOR2 Function and Localization

• NCOR2 (Nuclear receptor corepressor 2), also known as SMRT, is a transcriptional corepressor that promotes chromatin condensation to repress transcription of target genes. (PubMed:10077563, 10097068, 18212045) Related references: PMID:10077563, PMID:10097068, PMID:18212045
• It recruits histone deacetylases HDAC1, HDAC2, and HDAC3, and is required for activating HDAC3 enzymatic activity. (PubMed:22230954) Related references: PMID:22230954
• NCOR2 is involved in BCL6-dependent germinal center reactions, controlling B-cell proliferation and survival. (PubMed:18212045, 23911289) Related references: PMID:18212045, PMID:23911289
• Upon recruitment by ZBTB7A to androgen response elements, NCOR2 negatively regulates androgen receptor signaling and inhibits androgen-induced cell proliferation. (PubMed:20812024) Related references: PMID:20812024
• Multiple isoforms exist; isoform 1 and isoform 4 exhibit different affinities for various nuclear receptors.
• The protein is predominantly localized in the nucleus.
• It is ubiquitously expressed, with high levels detected in lung, spleen, and brain. (PubMed:10077563) Related references: PMID:10077563
• Post-translational modifications include acetylation, phosphorylation, methylation, and ubiquitination-like isopeptide bonds, which may regulate its activity and interactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-113), which may yield antibodies recognizing multiple isoforms. Validate isoform specificity if needed.
• For Western blotting, the predicted molecular weight is 274 kDa; use appropriate gel percentage and transfer conditions for high-molecular-weight proteins.
• In IHC-P, confirm nuclear staining pattern consistent with NCOR2 localization.
• As a polyclonal antibody, consider potential batch-to-batch variability and validate with appropriate controls.
• Cross-reactivity with mouse and rat has been indicated; verify in your specific model system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a reliable sourcing partner, providing diagnostic manufacturers and research labs with high-quality IVD raw materials. For projects investigating transcriptional regulation and chromatin remodeling, we offer validated monoclonal and polyclonal antibodies, including optimized pairs for ELISA and ancillary reagents. Contact us to explore bulk sourcing or rare-target material acquisition.