Anti-NCOR1 Rabbit Polyclonal Antibody for WB - O75376

Core Product Specifications and Parameters

Biological Background: NCOR1 Function and Localization

• Nuclear receptor corepressor 1 (NCOR1) mediates transcriptional repression by certain nuclear receptors, forming part of a complex that promotes histone deacetylation and repressive chromatin structures, thereby impeding access of basal transcription factors (PubMed:20812024). Related references: PMID:20812024
• NCOR1 participates in the transcriptional repressor activity produced by BCL6, a transcriptional regulator involved in lymphocyte differentiation and oncogenesis.
• Recruited by ZBTB7A to androgen response elements (AREs) on target genes, NCOR1 negatively regulates androgen receptor signaling and androgen-induced cell proliferation (PubMed:20812024). Related references: PMID:20812024
• NCOR1 mediates NR1D1-dependent repression and circadian regulation of TSHB expression, contributing to thyroid hormone homeostasis (By similarity).
• The NCOR1-HDAC3 complex is critical for circadian rhythm regulation, controlling expression of the core clock gene ARNTL/BMAL1 and lipid metabolism genes in the liver (By similarity).
• NCOR1 is predominantly localized to the nucleus, where it exerts its corepressor functions.
• Post-translational modifications include acetylation and phosphorylation, and the protein undergoes ubiquitin conjugation, as indicated by the presence of isopeptide bonds.
• Alternative splicing generates multiple isoforms, potentially contributing to functional diversity.

Experimental Guidance and Technical Tips

• The immunogen targets amino acids 1-200 at the N-terminus of NCOR1; consider verifying epitope accessibility in the experimental context.
• For Western blot, use low-percentage SDS-PAGE gels (e.g., 6%) and extended transfer times due to the large protein size (~270 kDa). Validate specificity using knockdown or knockout controls.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), antigen retrieval may enhance signal; optimize conditions for the tissue or cell type under study.
• As a polyclonal antibody, it may detect multiple NCOR1 isoforms; confirm the expected band pattern in relevant samples.
• When used for ELISA, pair with a suitable capture or detection antibody to improve assay performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides IVD manufacturers and research laboratories with comprehensive access to high-quality raw materials, including validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. For NCOR1-related studies in transcription regulation, circadian rhythms, or cancer biology, our one-stop sourcing capabilities extend to rare targets and custom development. Partner with CamelBio to streamline your supply chain from concept to clinic, ensuring reliable and consistent material support for your diagnostic and research applications.