Anti-NCOA7 Rabbit Monoclonal Antibody for WB, ELISA - Q8NI08

Core Product Specifications and Parameters

Biological Background: NCOA7 Function and Localization

• NCOA7 (Nuclear receptor coactivator 7) is also known as ERAP140, ESNA1, and TLDC4. It enhances transcriptional activities of nuclear receptors including ESR1, THRB, PPARG, and RARA. Related references: PMID:11971969
• The protein inhibits vacuolar-type ATPase (V-ATPase) by inducing disassembly of the complex, protecting the Golgi lumen from excess acidification and maintaining glycosylation enzyme conditions. Related references: PMID:38593795
• NCOA7 is involved in protection against oxidative damage and exhibits oxidation resistance activity, protecting neuronal cells from oxidative stress. Related references: PMID:17391516, PMID:25330068
• Isoforms with stronger neuroprotective activity also inhibit endosome-mediated viral entry by blocking viral-host membrane fusion and promoting lysosomal degradation of endocytosed material. Related references: PMID:30478388
• The protein localizes to the nucleus, cytoplasm, and Golgi apparatus (cis- and trans-Golgi network membranes), consistent with roles in transcription and membrane trafficking.
• It is highly expressed in brain, with weaker expression in mammary gland, ovary, uterus, prostate, stomach, bladder, spinal cord, pancreas, and cancer cell lines.
• Post-translational modifications include acetylation and phosphorylation; the protein features coiled-coil domains.
• The gene undergoes alternative splicing, producing isoforms with molecular weights ranging from 12 kDa to 106 kDa.

Experimental Guidance and Technical Tips

• The immunogen spans a region common to several NCOA7 isoforms; consider verifying reactivity with the isoform of interest in your experimental system.
• For Western blot, multiple bands between 12 kDa and 106 kDa may be observed due to alternative splicing; use appropriate positive controls such as brain or cancer cell lines.
• For ELISA, validate with recombinant protein or cell lysates; cross-reactivity with mouse allows studies in murine models.
• Optimize antibody dilution for each application and sample type; preliminary titration is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers reliable access to high-quality IVD raw materials like this NCOA7 rabbit monoclonal antibody, supporting research in nuclear receptor signaling, oxidative stress, and viral entry mechanisms. Our comprehensive supply capabilities include validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets. Partner with us to streamline your diagnostic development from concept to clinic.