Anti-NCOA4 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q13772

Core Product Specifications and Parameters

Biological Background: NCOA4 Function and Localization

• NCOA4 serves as a cargo receptor mediating ferritinophagy, the autophagic degradation of ferritin, which is critical for cellular iron homeostasis.
  Related references: PMID:25327288 PMID:26436293
• It targets the ferritin complex to autolysosomes upon starvation or iron depletion.
  Related references: PMID:25327288
• NCOA4 participates in erythropoiesis by regulating hemin-induced erythroid differentiation.
  Related references: PMID:26436293
• Exhibits transcriptional coactivator activity for androgen receptor (AR) and PPAR-gamma, though its AR coactivation is debated.
  Related references: PMID:10347167 PMID:8643607 PMID:10517667
• Associates with DNA replication origins and may inhibit origin activation by interacting with the MCM2-7 complex.
  Related references: PMID:24910095
• Localizes to autophagosomes, autolysosomes, cytoplasmic vesicles, nucleus, and chromosomes.
• Widely expressed across tissues, with a testis-specific isoform Beta.
• Keywords include proto-oncogene, chromosomal rearrangement (RET/PTC3 fusion in thyroid carcinoma), iron-sulfur cluster binding, and ubiquitination.

Experimental Guidance and Technical Tips

• For Western blot, consider using this antibody at an appropriate dilution to detect the ~70 kDa NCOA4 band in lysates from human, mouse, and rat tissues or cell lines.
• When performing IHC-P, optimize antigen retrieval and antibody concentration for the tissue type, and validate with adjacent control sections.
• For IF/ICC, include positive and negative controls to confirm specific localization to autophagic structures, nucleus, or cytoplasm.
• The immunogen (N-terminal 1-280 region) may contribute to cross-reactivity across these species, but confirm conservation in the target sequence.
• This polyclonal antibody may be applied in ELISA for quantitative detection; ensure proper coating and blocking conditions are established.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a streamlined, one-stop procurement experience for IVD raw materials. This anti-NCOA4 antibody supports research into iron homeostasis, autophagy, and nuclear receptor biology. Beyond individual antibodies, CamelBio provides validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing for rare target raw materials to accelerate your assay development from concept to clinic.