Anti-NCOA3 Rabbit Polyclonal Antibody for WB, IP, ELISA - Q9Y6Q9

Core Product Specifications and Parameters

Biological Background: NCOA3 Function and Localization

• NCOA3 (Nuclear Receptor Coactivator 3), also known as SRC-3, ACTR, AIB1, RAC3, pCIP, TRAM-1, and bHLHe42, is a key transcriptional coactivator that directly binds nuclear receptors and stimulates hormone-dependent gene expression.
• It serves as a central scaffold for multisubunit coactivator complexes, facilitating chromatin remodeling to enable transcriptional activation.
• NCOA3 coactivates a broad range of nuclear receptors, including steroid receptors (glucocorticoid and estrogen receptors), retinoid receptors (RARs and RXRs), thyroid hormone receptors, vitamin D3 receptor, and prostanoid receptors (PPARs).
• The protein possesses intrinsic histone acetyltransferase (HAT) activity, contributing to chromatin modification and epigenetic regulation of transcription.
• Beyond nuclear receptors, NCOA3 also participates in NF-kappa-B pathway coactivation through interaction with the NFKB1 subunit.
• Subcellular localization is both cytoplasmic and nuclear, consistent with its dynamic role in signal transduction and transcriptional regulation.
• Tissue expression is widespread, with highest levels in heart, skeletal muscle, pancreas, and placenta; lower expression in brain, lung, liver, and kidney.
• Post-translational modifications such as acetylation, methylation, and phosphorylation regulate NCOA3 stability, activity, and interactions.

Experimental Guidance and Technical Tips

• For Western blotting, note that NCOA3 has a predicted molecular weight of ~155 kDa; use appropriate gel percentage and transfer conditions for high-molecular-weight proteins.
• The immunogen is a synthetic peptide from the N-terminal region (aa 1-100) of human NCOA3; verify epitope accessibility in your sample system, especially if endogenous interaction partners may mask the epitope.
• Since this is a rabbit polyclonal antibody, it may recognize multiple isoforms of NCOA3; consider using isoform-specific controls if precise identification is required.
• For immunoprecipitation, optimize antibody concentration and incubation conditions for your lysate type; cross-reactivity is confirmed for human.
• ELISA applications may require a matched antibody pair or direct labeling; validate signal linearity with known amounts of recombinant protein or peptide standard.
• Always include appropriate positive and negative controls (e.g., NCOA3-overexpressing cells, knockout lysates) to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials and technical support, streamlining your journey from concept to clinic. This NCOA3 Rabbit pAb supports oncology, endocrinology, and chromatin biology research—backed by CamelBio's supply chain for validated antibody pairs, bulk ancillary reagents, and rare target sourcing. Partner with us for seamless procurement and dedicated consultation tailored to diagnostic development.