Anti-NCKAP1 Rabbit Polyclonal Antibody for WB, ELISA - Q9Y2A7

Core Product Specifications and Parameters

Biological Background: NCKAP1 Function and Localization

• Nck-associated protein 1 (NCKAP1), also designated HEM2, NAP1, NAP125, or p125Nap1, is a 128.8 kDa protein encoded by the NCKAP1 gene in humans.
• NCKAP1 is a core component of the WAVE regulatory complex, which promotes lamellipodia formation by regulating actin filament reorganization through the Arp2/3 complex, a process activated by the small GTPase RAC1.
• Within the WAVE1 complex, NCKAP1 is required for brain-derived neurotrophic factor (BDNF)-induced endocytic trafficking and signaling from early endosomes via the NTRK2 receptor.
• The protein localizes to the cell membrane and is enriched at the lamellipodium membrane, consistent with its role in cell motility.
• NCKAP1 is widely expressed across human tissues, with highest levels in brain, heart, and skeletal muscle; it is notably absent in peripheral blood leukocytes. Within the brain, NCKAP1 is found in Purkinje cells, the cerebellar dentate nucleus, hippocampal CA4 region and dentate gyrus, and frontal gray and white matter. Related references: PMID:28940097
• Post-translational modifications include acetylation, and alternative splicing generates multiple isoforms. UniProt keywords also highlight a transmembrane helix and potential host-virus interactions.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (aa 1-250) of human NCKAP1; cross-reactivity with mouse and rat suggests epitope conservation, but validation in each species is recommended.
• For Western blotting, the predicted molecular weight is ~129 kDa; consider using a low-percentage SDS-PAGE gel and extended transfer times to ensure efficient transfer of this high-molecular-weight protein.
• ELISA applications may require titration of the antibody and optimization of coating antigen concentrations; performance should be confirmed using recombinant NCKAP1 protein or cell lysates.
• As a polyclonal antibody, lot-to-lot consistency can vary; incorporating appropriate positive and negative controls is advised for reproducible results.

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CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials like validated NCKAP1 antibodies and related ancillary reagents. From antibody pairs to bulk monoclonal/polyclonal antibodies, our supply chain supports studies on actin cytoskeleton remodeling and neurological signaling. Reach out to leverage our sourcing expertise for rare targets and assay development.