Anti-NCK2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - O43639

Core Product Specifications and Parameters

Biological Background: NCK2 Function and Localization

• Cytoplasmic protein NCK2, also known as growth factor receptor-bound protein 4 (GRB4) or NCK adaptor protein 2 (Nck-2), is an SH2/SH3 domain-containing adapter protein.
• It associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates, linking receptor activation to downstream signaling pathways.
• NCK2 functions to maintain low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by protein phosphatase 1 (PP1), implicating a role in translation regulation.
• It plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling, contributing to gene expression control.
• Subcellularly, NCK2 is localized to the cytoplasm and endoplasmic reticulum.
• The protein is ubiquitously expressed across tissues, indicating a general housekeeping role.
• Post-translational modifications include acetylation and phosphorylation, which may modulate its interactions and functions.
• The molecular weight of NCK2 is approximately 43 kDa (42915 Da).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human NCK2 protein (amino acids 1–380), which may enable recognition of multiple epitopes and robust detection in various assays.
• For Western blot, consider a starting primary antibody dilution and optimize based on target abundance in your sample; a band around 43 kDa is expected.
• In IHC-P, antigen retrieval methods and antibody titration are recommended to achieve optimal staining with minimal background.
• For ELISA, the antibody can be used as a detection reagent; pair with appropriate capture antibodies and validate in the relevant sample matrix.
• Cross-reactivity with mouse and rat NCK2 has been reported, but we advise confirming specificity in your experimental system.
• Ensure proper storage and handling (shipped on ice bag) to maintain antibody integrity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to IVD raw materials, including this anti-NCK2 antibody for signal transduction and translation regulation studies. As a dedicated sourcing partner, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom raw-material sourcing to accelerate your development from concept to clinic.