Anti-NCK1 Rabbit Polyclonal Antibody for WB and ELISA - P16333

Core Product Specifications and Parameters

Biological Background: NCK1 Function and Localization

• NCK1 (Nck-1) is an SH2/SH3 adapter protein, also known as NCK, NCKalpha, or cytoplasmic protein NCK1.
• It associates with tyrosine-phosphorylated growth factor receptors (e.g., KDR, PDGFRB) or their substrates, linking receptor activation to downstream signaling.
• NCK1 maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by protein phosphatase 1 (PP1).
• The protein is involved in the DNA damage response, facilitating efficient activation of downstream effectors like CHEK2, though not directly sensing damage.
• It modulates the activation of EIF2AK2/PKR by dsRNA, implicating it in translation regulation.
• NCK1 plays a role in ELK1-dependent transcriptional activation downstream of activated Ras signaling.
• Subcellularly, NCK1 localizes to the cytoplasm, endoplasmic reticulum, and nucleus, reflecting its multifaceted functions.
• The protein contains one SH2 domain and three SH3 domains, characteristic of adapter proteins mediating protein-protein interactions.

Experimental Guidance and Technical Tips

• This antibody is raised against a recombinant protein covering amino acids 58-377 of human NCK1. Researchers should validate reactivity in their specific experimental systems, as the epitope may be influenced by protein conformation or PTMs.
• For WB, a band near 43 kDa is expected, but alternative splicing or post-translational modifications may produce additional bands.
• When using ELISA, the recombinant immunogen can serve as a positive control to confirm antibody specificity.
• Given cross-reactivity with mouse, this antibody can be considered for studies in mouse models, but it is advisable to verify performance in target tissues.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw materials, offering a complete portfolio of validated antibodies, recombinant proteins, and ancillary reagents. This anti-NCK1 polyclonal antibody supports research into growth factor signaling, DNA damage response, and cell adhesion pathways. From optimized antibody pairs to rare target sourcing, CamelBio streamlines your diagnostic development from concept to clinic.