Anti-NCK1 Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P16333

Core Product Specifications and Parameters

Biological Background: NCK1 Function and Localization

• NCK1 is an SH2/SH3 adapter protein, also known as NCK, nck-1, or NCKalpha, encoded by the NCK1 gene; alternative splicing produces multiple isoforms.
• It associates with tyrosine-phosphorylated growth factor receptors (e.g., KDR, PDGFRB) and their substrates, linking receptor activation to downstream signaling cascades.
• NCK1 maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by protein phosphatase 1 (PP1), thereby regulating translation initiation.
• It participates in the DNA damage response, facilitating efficient activation of downstream effectors like CHEK2, downstream of ATM/ATR damage sensors.
• The protein modulates EIF2AK2/PKR activation by double-stranded RNA, influencing the integrated stress response.
• NCK1 interacts with ephrin receptors, potentially contributing to cell adhesion and migration processes.
• Subcellular localization includes the cytoplasm, endoplasmic reticulum, and nucleus, consistent with its roles in signal transduction and gene expression.
• Post-translational modifications include acetylation and phosphorylation, which may modulate its interactions and functions; the protein has a calculated molecular weight of ~43kDa.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from amino acids 200-300 of human NCK1; consider using a positive control expressing full-length NCK1 protein for validation.
• For Western blot, detect a band at approximately 43kDa in lysates from human, mouse, or rat cell lines; validate specificity with peptide blocking or knockdown/knockout controls.
• In immunohistochemistry (paraffin-embedded sections), antigen retrieval may be necessary; optimize staining conditions for each tissue type.
• For immunofluorescence/ICC, fixation and permeabilization conditions should be optimized to preserve epitope accessibility and cellular architecture.
• ELISA applications may benefit from pairing with a suitable detection antibody; consider checkerboard titrations for optimal signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with a reliable, one-stop platform for sourcing IVD raw materials, from validated antibody pairs to bulk ancillary reagents. This NCK1 monoclonal antibody supports studies in cell signaling, DNA damage, and cancer biology, addressing critical pathways for diagnostic development. Partner with CamelBio to streamline your procurement from concept to clinic, ensuring a stable supply of high-quality reagents for your assay needs.