Anti-NCF4/p40-phox Polyclonal Antibody for WB, ELISA - Q15080

Core Product Specifications and Parameters

Biological Background: NCF4/p40-phox Function and Localization

• NCF4 (Neutrophil cytosol factor 4), also known as p40-phox, SH3PXD4, or NCF, is a 39032 Da protein encoded by the NCF4 gene on human chromosome 22. It is predominantly expressed in hematopoietic cells.
• p40-phox is a regulatory subunit of the phagocyte NADPH oxidase complex, which generates superoxide anion (O2(-)) by transferring electrons from cytosolic NADPH to molecular oxygen, crucial for microbial killing in phagocytes.
• The NADPH oxidase activation involves assembly of cytosolic subunits (including p40-phox, p47-phox, p67-phox) with the membrane-bound flavocytochrome b558 (gp91-phox and p22-phox) upon cell stimulation.
• p40-phox contains an N-terminal PX domain that binds phosphatidylinositol 3-phosphate, and a C-terminal SH3 domain, mediating protein-lipid and protein-protein interactions essential for oxidase activation and membrane targeting.
• Subcellularly, p40-phox is found in the cytosol of resting cells and translocates to the endosome and phagosomal membranes during activation, facilitating localized ROS production.
• Alternative splicing produces isoforms, and mutations in NCF4 are associated with autosomal recessive chronic granulomatous disease (CGD), characterized by recurrent infections.
• The protein is phosphorylated on particular residues in response to stimuli such as phorbol ester, enhancing its binding affinity to membranes and contributing to oxidase complex assembly.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 1-190 of human NCF4/p40-phox (NP_038202.2), encompassing the PX domain. Validate antibody specificity for the intended application in the relevant sample system.
• For Western blot (WB), NCF4/p40-phox has a predicted molecular weight of approximately 39 kDa. Confirm migration patterns using appropriate positive controls and consider potential post-translational modifications.
• The antibody is also suitable for ELISA, likely recognizing the native protein. Titration and optimization are recommended for each experimental setup.
• Because cross-reactivity is reported for human, mouse, and rat, consider this antibody for detecting p40-phox across these species, but verify reactivity in your specific models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a streamlined supply chain for IVD raw materials and technical services from concept to clinic. For the NCF4/p40-phox target, we provide validated polyclonal antibodies, bulk ancillary reagents, and support for rare target raw-material sourcing. Partner with CamelBio to accelerate your phagocyte oxidase research and assay development with reliable, high-quality materials.