Anti-NCF4/p40-phox Monoclonal Antibody for WB, IHC-P, IP, ELISA - Q15080

Core Product Specifications and Parameters

Biological Background: NCF4/p40-phox Function and Localization

• NCF4/p40-phox is a subunit of the phagocyte NADPH oxidase complex that generates superoxide anions by transferring electrons from NADPH to molecular oxygen.
• The protein contains SH3 and PX domains, classifying it as an SH3 and PX domain-containing protein 4 (SH3PXD4); it is also known as neutrophil NADPH oxidase factor 4.
• Activation of the complex requires assembly of cytosolic subunits, including NCF4, with the membrane-bound core complex; this process is initiated by phosphorylation-dependent binding of NCF1/p47-phox to CYBA/p22-phox.
• NCF4 expression is restricted to hematopoietic cells.
• Subcellularly, it localizes to the cytoplasm and cytosol, and associates with endosome membranes and the plasma membrane upon activation.
• The protein is subject to phosphorylation and alternative splicing, and is implicated in chronic granulomatous disease when defective.
• The molecular weight of the canonical isoform is approximately 39 kDa.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 240-339, located in the C-terminal region of human NCF4. This region includes part of the PX domain and adjacent sequences, so the antibody may recognize full-length NCF4 and potentially splice variants containing this segment.
• For Western blotting, a band near 39 kDa is expected under reducing conditions; consider using hematopoietic cell lysates (e.g., HL-60, neutrophils) as positive controls.
• In immunohistochemistry, paraffin-embedded sections may require heat-induced epitope retrieval; antibody dilution and incubation conditions should be optimized on known positive tissues such as spleen or bone marrow.
• For immunoprecipitation, the rabbit monoclonal format often provides high affinity, but co-immunoprecipitation of the NADPH oxidase complex components may require mild lysis conditions to maintain protein-protein interactions.
• In ELISA, the antibody raised against the peptide immunogen may be used for detecting recombinant or native NCF4; pairing with a different epitope-specific antibody could enable sandwich ELISA development.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, offering reliable access to antibodies like this NCF4/p40-phox monoclonal antibody for research on phagocyte respiratory burst and chronic granulomatous disease. We support diagnostic developers with validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and specialized sourcing of rare target raw materials to streamline your assay development.