Anti-NCBP2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P52298

Core Product Specifications and Parameters

Biological Background: NCBP2 Function and Localization

• NCBP2 (also known as CBP20, NIP1, CBC2, PIG55) is the 20 kDa subunit of the nuclear cap-binding complex (CBC), which binds co-transcriptionally to the 5' cap of pre-mRNAs and is essential for multiple aspects of RNA metabolism.
• As part of the CBC, NCBP2 participates in pre-mRNA splicing, mRNA export (including U snRNA and intronless mRNA export), pioneer round translation, nonsense-mediated mRNA decay (NMD), and microRNA biogenesis.
• The CBC complex is critical for the pioneer round of translation, during which NMD takes place; it is later replaced by eIF4E for steady-state translation.
• NCBP2 directly recognizes and binds the m7GpppG cap structure, but requires NCBP1/CBP80 to stabilize the high-affinity cap-binding conformation.
• The conventional cap-binding complex with NCBP2 binds both small nuclear RNA (snRNA) and messenger RNA (mRNA) and is involved in their export from the nucleus. Related references: PMID:26382858
• NCBP2 localizes to both the nucleus and cytoplasm, reflecting its roles in nuclear RNA processing and cytoplasmic translation regulation.
• Post-translational modifications include acetylation, phosphorylation, and methylation, which may modulate its activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-156 of NCBP2, covering the N-terminal region; the antibody is expected to recognize the full-length protein.
• For Western blot, the predicted molecular weight of NCBP2 is ~18 kDa; consider using a 12% SDS-PAGE gel and PVDF membrane for optimal transfer and detection.
• In immunofluorescence/ICC, NCBP2 shows both nuclear and cytoplasmic staining; validate the subcellular distribution in your specific cell type using appropriate fixation and permeabilization conditions.
• ELISA may be employed for quantitative detection of NCBP2 in cell lysates or serum samples; include negative and positive controls to ensure specificity.
• As the antibody recognizes both human and mouse NCBP2, it can be used in cross-species applications, but always confirm reactivity in your sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw material procurement, offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents to streamline diagnostic development. This NCBP2 antibody supports research into RNA processing, cap-binding complex biology, and related diseases, making it a valuable addition to your assay toolkit. Leverage CamelBio’s expertise to source rare-target raw materials and accelerate your project from concept to clinic.