Anti-NCAPH Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q15003

Core Product Specifications and Parameters

Biological Background: NCAPH Function and Localization

• NCAPH encodes the regulatory condensin complex subunit 2 (also known as hCAP-H, CAP-H, BRRN1), which is essential for mitotic chromosome condensation and segregation.
• The condensin complex, containing NCAPH, introduces positive supercoils into DNA and cooperates with topoisomerases to compact chromatin into mitotic chromosomes (PubMed:11136719). Related references: PMID:11136719
• In neural stem cells, NCAPH ensures accurate chromosome condensation during early neurogenesis, and loss-of-function mutations lead to primary microcephaly type 23 (MCPH23) via reduced neuron production (PubMed:27737959). Related references: PMID:27737959
• Subcellularly, NCAPH localizes to the cytoplasm and nucleus, and associates with chromosomes specifically during mitosis.
• The protein is subject to multiple post-translational modifications including acetylation, phosphorylation, and ubiquitination (isopeptide bond formation), likely regulating its mitotic functions.
• NCAPH is widely expressed at low levels across tissues, with higher expression in proliferating cells, consistent with its role in cell division.
• Alternative splicing of the NCAPH gene produces multiple transcript variants, potentially yielding isoforms with functional differences.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 1‑250 (N‑terminal region) of human NCAPH; consider that the antibody recognizes epitopes within this domain and may detect isoforms containing this sequence.
• For Western blotting (WB), a band around 83 kDa is expected in human cell lysates; validate in a relevant sample system (e.g., HeLa, HEK293) and optimize loading and transfer conditions.
• In immunofluorescence/immunocytochemistry (IF/ICC), NCAPH displays nuclear and cytoplasmic staining; use appropriate fixation/permeabilization protocols to preserve epitope accessibility and verify co‑localization with mitotic chromosomes.
• For ELISA, the antibody can be used as a detection reagent; pair with a suitable capture antibody if developing a sandwich assay, and test using recombinant NCAPH protein or cell lysates.
• As a polyclonal antibody, lot‑to‑lot consistency and cross‑reactivity with closely related condensin subunits may vary; confirm specificity in your application via knockdown/knockout controls or comparing with known expression patterns.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw-material sourcing, offering a comprehensive portfolio that includes this NCAPH polyclonal antibody alongside validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. Whether you are developing chromosome biology research tools or clinical diagnostic assays, our one‑stop platform helps streamline procurement from concept to clinic with reliable supply chain support.