Anti-NCAM/CD56 Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P13595

Core Product Specifications and Parameters

Biological Background: NCAM/CD56 Function and Localization

• NCAM1 (CD56) is a neural cell adhesion molecule belonging to the immunoglobulin superfamily, involved in homophilic and heterophilic cell-cell interactions.
• It plays critical roles in neuronal development, including neurite outgrowth, fasciculation, and axon guidance.
• Multiple isoforms (e.g., 120, 140, and 180 kDa) are generated by alternative splicing, with varying cytoplasmic domains or GPI-anchor attachment.
• The protein is primarily localized at the cell membrane; some isoforms are single-pass transmembrane proteins, while others are GPI-anchored.
• Post-translational modifications such as glycosylation, phosphorylation, and disulfide bond formation modulate its adhesive properties and signaling.
• NCAM also contains heparin-binding domains, enabling interactions with extracellular matrix components.
• The extracellular region is composed of five immunoglobulin-like domains and two fibronectin type III repeats, typical of cell adhesion molecules.

Experimental Guidance and Technical Tips

• For Western blot, multiple isoforms ranging from 67 to 119 kDa may be detected due to alternative splicing and post-translational modifications; validate in the relevant sample system.
• In immunofluorescence and immunohistochemistry, fixation and permeabilization conditions can affect epitope accessibility; consider optimizing antigen retrieval for paraffin-embedded sections.
• For ELISA, the antibody may serve as a capture or detection reagent; perform cross-reactivity and specificity tests to confirm suitability for the intended assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one-stop access to IVD raw materials, technical services, and consulting—from concept to clinic. Our NCAM/CD56 Rabbit Monoclonal Antibody supports neuroscience and cell adhesion research, and we also supply validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets. Let CamelBio streamline your next project with reliable, scalable supply solutions.