Anti-NCAM1/CD56 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P13591

Core Product Specifications and Parameters

Biological Background: NCAM1 / CD56 Function and Localization

• Neural cell adhesion molecule 1 (NCAM1), also known as CD56, is a member of the immunoglobulin superfamily of cell adhesion molecules.
• NCAM1 mediates homophilic adhesion between neurons, contributing to neurite fasciculation and outgrowth, which are essential for neural development and plasticity.
• The protein undergoes extensive alternative splicing, generating multiple isoforms that differ in their membrane anchorage; some are transmembrane while others are attached via a glycosylphosphatidylinositol (GPI) anchor.
• NCAM1 is primarily located at the cell membrane, but soluble forms can be secreted into the extracellular space.
• It is a glycoprotein with multiple N-linked glycosylation sites and contains disulfide bonds that stabilize its immunoglobulin-like domains.
• NCAM1 serves as a receptor for several viruses, including rabies virus and Zika virus, facilitating host cell entry.
• Post-translational modifications include phosphorylation, which may regulate its adhesive functions and signaling.
• The predicted molecular weight of the canonical isoform is approximately 94.6 kDa, but glycosylation increases the apparent weight to about 95 kDa on SDS-PAGE.

Experimental Guidance and Technical Tips

• When performing western blotting, use denaturing and reducing conditions; expect a specific band near 95 kDa corresponding to the glycosylated full-length NCAM1 protein.
• For immunohistochemistry on paraffin-embedded sections, antigen retrieval may be necessary; consider testing on neural tissues or NCAM1-expressing tumors.
• As the immunogen covers a cytoplasmic region (amino acids 759–858), the antibody is expected to recognize all membrane-anchored isoforms regardless of extracellular domain splice variants.
• If utilizing the antibody in viral infection studies, validate it in relevant cell lines known to express NCAM1 and confirm receptor functionality through appropriate controls.
• Due to reported cross-reactivity with mouse and rat, include species-matched positive and negative controls to confirm specificity in your experimental setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research institutes by providing a comprehensive range of IVD raw materials, from validated antibody pairs to bulk ancillary reagents. This anti-NCAM1/CD56 polyclonal antibody is ideal for neuroscience and virology research applications, and we can assist in sourcing matched detection antibodies, recombinant proteins, and other related raw materials. With a focus on bridging from concept to clinic, our team offers tailored procurement solutions for even the most challenging targets.