Anti-NCAM1/CD56 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P13591

Core Product Specifications and Parameters

Biological Background: NCAM1/CD56 Function and Localization

• NCAM1 (Neural cell adhesion molecule 1, CD56) is a cell adhesion molecule that mediates neuron-neuron adhesion, promotes neurite fasciculation, and stimulates neurite outgrowth.
• It serves as a receptor for microbial infections, specifically for rabies virus and Zika virus, facilitating viral entry into host cells.
• The protein undergoes extensive alternative splicing, yielding multiple isoforms: some are single-pass type I membrane proteins, others are attached to the membrane via a GPI anchor, and a secreted form also exists.
• Subcellular localization includes various cell membrane compartments (cell membrane, membrane) and secreted forms, reflecting isoform diversity.
• Key post-translational modifications include glycosylation, phosphorylation, and disulfide bond formation, as indicated by keywords in UniProt.
• The NCAM family includes immunoglobulin domain-containing adhesion molecules; NCAM1 features five N-terminal Ig-like domains and two fibronectin type-III repeats.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 759-858 near the C-terminal region of the NCAM1 isoform (NP_851996.2). When detecting specific isoforms, consider validating in the relevant sample system using isoform-specific controls.
• For Western blot (WB), the predicted protein weight is 95 kDa; however, due to glycosylation variants and alternative splicing, multiple bands may appear. Optimize loading and electrophoresis conditions accordingly.
• In IHC-P and IF/ICC, tissue fixation and antigen retrieval methods can influence staining. Validate with appropriate controls, including negative isotype controls and tissue known to express CD56 (e.g., neural tissue, NK cells).
• ELISA applications may require titration of the antibody and antigen to establish optimal working concentrations; consider using recombinant NCAM1 as a positive control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement by offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. For NCAM1/CD56 research, we provide comprehensive supply solutions to support diagnostic and neuroscience workflows—from concept to clinic.