Anti-NCAM (CD56) Rabbit PolymAb® Monoclonal Antibody for WB, IF/ICC, IF-P, IHC-P, ELISA - P13591 / P1595

Core Product Specifications and Parameters

Biological Background: NCAM1 Function and Localization

• NCAM1 (Neural cell adhesion molecule 1), also known as CD56 and MSK39, is a glycoprotein of the immunoglobulin superfamily that mediates cell–cell adhesion through homophilic and heterophilic interactions.
• It plays a critical role in nervous system development, including neuron-neuron adhesion, neurite fasciculation, and promotion of neurite outgrowth.
• NCAM1 is expressed as multiple isoforms via alternative splicing, including transmembrane and glycosylphosphatidylinositol (GPI)-anchored variants, with a calculated molecular mass of approximately 94.6 kDa (mature protein ~95–120 kDa due to glycosylation).
• Subcellularly, NCAM1 localizes predominantly to the cell membrane as a single-pass type I membrane protein, but secreted and GPI-anchored forms also exist.
• Post-translational modifications include N-linked glycosylation, disulfide bond formation, and phosphorylation on cytoplasmic residues; the GPI-anchored isoform is lipidated.
• NCAM1 has been identified as a receptor for viral entry; it acts as a host cell receptor for rabies virus and Zika virus.
• It is widely used as a marker for natural killer cells and certain neuroendocrine tumors in diagnostic immunohistochemistry.

Experimental Guidance and Technical Tips

• The immunogen covers residues 20–413 of human/mouse CD56, corresponding to the extracellular immunoglobulin-like domains. The antibody likely recognizes surface‑exposed epitopes, making it suitable for live‑cell IF/ICC and flow cytometry.
• For Western blot, expect bands around 95–120 kDa due to glycosylation; validate using appropriate positive control cell lines (e.g., brain tissue, NK cells).
• In IHC-P, antigen retrieval (e.g., citrate buffer, pH 6.0) may be required; test both membrane and cytoplasmic staining patterns depending on isoform expression.
• Cross-reactivity with rat has been reported, but it is advisable to verify in your specific tissue or cell system.
• For ELISA, the antibody can be used as a capture or detection reagent after confirming specificity with recombinant NCAM protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, contract research organizations, and clinical laboratories with a reliable, one‑stop supply of high‑quality IVD raw materials. This NCAM antibody is part of our extensive portfolio of validated neural and immune‑cell markers, supporting neuroscience and oncology diagnostic development. Beyond single antibodies, we offer matched antibody pairs, bulk recombinant proteins, and custom sourcing for rare‑target reagents—backed by technical consulting to help move your assay from concept to clinic smoothly.