Anti-NCALD Rabbit Polyclonal Antibody for WB and ELISA - P61601

Core Product Specifications and Parameters

Biological Background: NCALD Function and Localization

• Neurocalcin-delta is a calcium-binding protein encoded by the NCALD gene (UniProt P61601).
• It binds three calcium ions and may function as a calcium sensor involved in the regulation of rhodopsin phosphorylation.
• The protein is prominently expressed in neuronal tissues including the cerebrum, cerebellum, brain stem, and spinal cord, as well as in retina, testis, ovary, and small intestine.
• Neurocalcin-delta is a lipoprotein that undergoes N-terminal myristoylation, facilitating its association with cellular membranes.
• It contains calcium-binding EF-hand repeats, characteristic of the neuronal calcium sensor family.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 1-193) corresponds to the N-terminal region of human NCALD, which is highly conserved across human, mouse, and rat, consistent with the reported cross-reactivity.
• For Western blotting, consider using lysates from retina or brain tissues, where NCALD expression is highest; verify the expected band at 22 kDa.
• In ELISA applications, coat with recombinant NCALD protein and titrate the antibody to determine optimal concentrations for sensitive detection.
• Validate the antibody in your specific sample system and application; cross-reactivity with other neuronal calcium sensors should be assessed by sequence alignment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials like this NCALD polyclonal antibody. Our portfolio includes validated antibody pairs, optimized polyclonal antibodies, and ancillary reagents to support neuroscience and calcium signaling studies. Leverage our integrated supply chain to accelerate your assay development from concept to clinic.