Anti-NBS1/NBN Rabbit Polyclonal Antibody (KO Validated) for WB, IHC-P, ELISA - O60934

Core Product Specifications and Parameters

Biological Background: Nibrin Function and Localization

• NBN is a component of the MRN complex (MRE11-RAD50-NBN), which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, and meiosis. Related references: PMID:10888888 PMID:15616588 PMID:18411307

• The MRN complex mediates end resection of damaged DNA to generate single-stranded DNA for homologous recombination; it also recruits other repair factors and activates ATM and ATR kinases upon DNA damage. Related references: PMID:19759395 PMID:28867292 PMID:9705271

• Within the MRN complex, NBN acts as a protein-protein adapter, specifically recognizing phosphorylated proteins and promoting their recruitment to DNA damage sites, including MRE11, RAD50, ATM, ATR, and RBBP8/CtIP. Related references: PMID:12419185 PMID:15616588 PMID:18411307 PMID:18583988 PMID:15064416 PMID:15790808 PMID:19759395 PMID:27814491 PMID:27889449 PMID:30787182 PMID:31537797

• NBN is involved in telomere length maintenance through interaction with TERF2; its phosphorylation by CDK2 promotes non-homologous end joining repair at telomeres, while unphosphorylated NBN favors microhomology-mediated end-joining. Related references: PMID:10888888 PMID:28216226

• NBN enhances AKT1 phosphorylation, possibly by associating with the mTORC2 complex. Related references: PMID:23762398

• Subcellularly, NBN localizes to the nucleus, chromosomes, PML bodies, and telomeres.

• NBN is ubiquitously expressed, with relatively high levels in testis. Related references: PMID:9590180

• Post-translational modifications include phosphorylation and ubiquitination (isopeptide bond formation).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 520–754) of human NBN; consider its recognition of full-length and possibly truncated forms in your assays.
• For western blotting, a band around 85 kDa is expected; however, confirm molecular weight in your sample system and consider potential post-translational modifications that may shift migration.
• In immunohistochemistry (IHC-P), validate staining in tissues known to express NBN, such as testis; optimize antigen retrieval and antibody dilution for your specific sample type.
• The antibody cross-reacts with mouse and rat NBN; verify species-specific reactivity and signal specificity in your model system.
• For ELISA applications, test the antibody with recombinant NBN protein or peptide coating, and establish appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted partner for IVD raw materials, providing a one-stop sourcing bridge from discovery to commercialization. With this anti-NBS1/NBN polyclonal antibody, we support your DNA damage response, telomere biology, and oncology research. Beyond antibodies, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and assistance in sourcing rare-target raw materials—all backed by expert technical support to accelerate your diagnostic development journey.