Anti-NBS1/NBN Polyclonal Antibody for WB, IF/ICC, ELISA - O60934

Core Product Specifications and Parameters

Biological Background: Nibrin (NBS1/NBN) Function and Localization

• Nibrin (NBN), also known as p95 or Nijmegen breakage syndrome protein 1, is a key component of the MRN complex, which plays a central role in double-strand break repair, DNA recombination, meiotic recombination, and telomere maintenance. Related references: PMID:10888888, PMID:15616588, PMID:18411307
• The MRN complex facilitates end resection of damaged DNA, a critical step for homologous recombination, through the exonuclease and endonuclease activities of MRE11. Related references: PMID:19759395, PMID:28867292, PMID:9705271
• NBN functions as a protein adapter, recognizing and binding phosphorylated proteins to recruit DNA repair factors such as MRE11, RAD50, ATM, and ATR to damage sites. Related references: PMID:12419185, PMID:15064416, PMID:15616588, PMID:15790808, PMID:18411307, PMID:18583988
• NBN mediates the recruitment of phosphorylated RBBP8/CtIP to double-strand breaks, enabling cooperative end resection and the removal of protein-adducted DNA ends. Related references: PMID:19759395, PMID:27814491, PMID:27889449
• At telomeres, NBN interacts with TERF2 to regulate length maintenance and DNA repair pathway choice; phosphorylation by CDK2 promotes non-homologous end joining, while unphosphorylated NBN favors microhomology-mediated end-joining. Related references: PMID:10888888, PMID:28216226
• NBN enhances AKT1 phosphorylation, potentially through association with the mTORC2 complex. Related references: PMID:23762398
• Ubiquitously expressed with high levels in testis, NBN is essential for genomic stability, and mutations cause Nijmegen breakage syndrome. Related references: PMID:9590180

Experimental Guidance and Technical Tips

• The immunogen sequence (aa 650–750) is located in the C-terminal region of human NBN; antibodies may recognize epitopes in this conserved region.
• For WB, expect a band at approximately 85 kDa; whole-cell lysates from human, mouse, or rat origin are suitable starting samples.
• In IF/ICC, staining is expected in the nucleus and at DNA damage foci; appropriate fixation and permeabilization methods should be optimized.
• For ELISA, consider using the immunizing peptide or recombinant NBN protein as a coating antigen to verify antibody binding under non-denaturing conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw material sourcing, offering this high-quality anti-NBS1/NBN polyclonal antibody for DNA damage repair and genome stability research. Our comprehensive portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to support your assay development. For rare targets or custom procurement needs, our expert team can source tailored raw materials to accelerate your project from concept to clinic.