Anti-Nav1.5/SCN5A Monoclonal Antibody for WB, IHC-P, ELISA - Q14524

Core Product Specifications and Parameters

Biological Background: Nav1.5 Function and Localization

• Nav1.5, encoded by the SCN5A gene, is the pore-forming alpha subunit of the cardiac voltage-gated sodium channel, also known as sodium channel protein cardiac muscle subunit alpha or hH1.
• It directly mediates the depolarizing phase of action potentials by enabling selective Na+ influx, a process essential for membrane excitability and electrical signal propagation. Related references: PMID:33712541 PMID:40768348 PMID:1309946 PMID:21447824 PMID:23085483
• As the predominant sodium channel in myocardial cells, Nav1.5 initiates the heartbeat by driving the upstroke of the cardiac action potential. Related references: PMID:11234013 PMID:11804990 PMID:12569159
• Nav1.5 is required for normal electrical conduction, including formation of the infranodal ventricular conduction system, through interaction with XIRP2.
• Subcellularly, Nav1.5 localizes to the sarcolemma, T-tubules, cell membrane, perinuclear cytoplasm, and cell junctions.
• Tissue expression is predominantly in atrial and ventricular cardiac muscle, with additional detection in jejunal smooth muscle; isoform 4 is brain-specific.
• Mutations in SCN5A are associated with Brugada syndrome, long QT syndrome type 3, atrial fibrillation, and cardiomyopathy; post-translational modifications include methylation, phosphorylation, ubiquitination, and glycosylation.

Experimental Guidance and Technical Tips

• For Western blotting, anticipate multiple bands around 227 kDa, 223 kDa, 221 kDa, or 225 kDa due to glycosylation or isoform variations; validate detection using appropriate positive and negative controls.
• In immunohistochemistry (paraffin-embedded) experiments, optimize antigen retrieval conditions and antibody dilution based on your specific tissue type; cardiac muscle sections are a recommended positive control.
• This antibody can be used in ELISA for quantitative or qualitative detection of human Nav1.5; consider direct or indirect coating strategies and verify cross-reactivity with your sample matrix.
• The immunogen corresponds to amino acids 273–357 of human Nav1.5; confirm that the epitope region is accessible in your assay format and target conformation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one-stop access to IVD raw materials, including validated antibody pairs and monoclonal antibodies like this anti‑Nav1.5/SCN5A clone, optimized for cardiac ion‑channel applications. Our portfolio spans bulk ancillary reagents and the sourcing of rare‑target raw materials, supporting the entire development pipeline from concept to clinic. Partner with CamelBio to streamline your procurement and accelerate assay development for cardiovascular disorders.