Anti-Natriuretic peptides A Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P01160

Core Product Specifications and Parameters

Biological Background: Natriuretic Peptides A Function and Localization

• Natriuretic peptides A (NPPA), also known as atrial natriuretic factor (ANF) or proANF, encodes a preproprotein proteolytically processed into multiple active peptides, including atrial natriuretic peptide (ANP), long-acting natriuretic peptide, vessel dilator, and urodilatin, each with distinct cardio-renal functions.
• ANP, the major circulating form, binds to NPR1 to stimulate cGMP production, leading to vasodilation, natriuresis, diuresis, and inhibition of aldosterone synthesis, thereby regulating blood pressure and fluid-electrolyte balance. Related references: PMID:1660465, PMID:1672777, PMID:21098034, PMID:2532366, PMID:2825692, PMID:7595132
• Beyond classical renal effects, ANP inhibits cardiac remodeling and hypertrophy by inducing cardiomyocyte apoptosis and attenuating cardiomyocyte and fibroblast growth, highlighting a protective cardiovascular role. Related references: PMID:16875975
• In adipose tissue, ANP promotes lipid metabolism and energy expenditure via cGMP/PKG pathways, activating AMPK and UCP1-dependent thermogenesis in brown fat, linking cardiovascular and metabolic regulation. Related references: PMID:15741263, PMID:18835931, PMID:21672517
• The NPPA prohormone also gives rise to long-acting natriuretic peptide, vessel dilator, and urodilatin, each demonstrating vasodilatory and natriuretic activities through distinct receptors and mechanisms. Related references: PMID:2162527, PMID:2825692, PMID:7595132, PMID:7720651, PMID:11145122, PMID:2532366, PMID:7955907
• The protein is secreted and detected in blood, urine, and various tissues, with highest expression in atrial cardiomyocytes and kidney distal tubular cells; it is also found in brain gray matter and adipocytes.
• Post-translational modifications include phosphorylation and disulfide bond formation; genetic variants in NPPA are associated with atrial fibrillation and cardiomyopathy.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 26-151 of human NPPA (propeptide region), suggesting the antibody may recognize multiple NPPA-derived peptides (proANF, ANP, etc.). Validate detection of specific forms in relevant sample systems.
• For Western blot, use reducing conditions; expect a band around 16 kDa for proANF, with possible smaller bands representing processed peptides. Optimize protein loading and transfer conditions.
• For immunohistochemistry/immunofluorescence, use fixed and permeabilized tissue sections from heart or kidney; antigen retrieval may be required. Validate staining specificity with positive and negative controls.
• Given cross-reactivity with mouse and rat, the antibody is suitable for rodent models; verify performance in these species with appropriate tissue lysates.
• The antibody is shipped under cold conditions (ice bag); upon receipt, aliquot and store at -20 °C. Avoid repeated freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive sourcing solutions for IVD raw materials, offering high-quality antibodies, validated antibody pairs, and ancillary reagents tailored to cardiovascular biomarker development. This anti-Natriuretic peptides A antibody supports research and assay design in blood pressure regulation, heart failure, and renal function studies. With an integrated supply chain and technical expertise, we accelerate your transition from concept to clinical application, covering every stage from discovery to diagnostic deployment.