Anti-NAT9 Rabbit Polyclonal Antibody for WB and ELISA - Q9BTE0

Core Product Specifications and Parameters

Biological Background: NAT9 Function and Localization

• NAT9 (also known as EBSP, hNATL) encodes an alpha/beta-tubulin-N-acetyltransferase that specifically acetylates the N-terminal residues of alpha- and beta-tubulin, a modification linked to microtubule stability and dynamics.
• The protein belongs to the N-acetyltransferase family, functioning as an acyltransferase, and may contribute to post-translational regulation of the cytoskeleton.
• NAT9 is alternatively spliced, generating multiple transcript variants, which might impact expression or functional diversity across different tissues.
• Originally identified as an embryo brain-specific protein, NAT9 likely plays a role in neurodevelopment, though its precise tissue distribution remains to be fully characterized.
• Subcellular localization data are limited; however, given its tubulin acetylation activity, NAT9 may localize to the cytoplasm or microtubule-associated compartments.
• Acetylation of tubulin is a critical post-translational modification that influences cell motility, division, and neuronal function, implying NAT9’s relevance in both normal physiology and disease contexts.
• The protein has a predicted molecular weight of 23.4 kDa and is classified under keywords such as acyltransferase, proteomics identification, and reference proteome.

Experimental Guidance and Technical Tips

• The immunogen corresponds to full-length human NAT9, so the antibody is expected to detect the native protein at around 23 kDa in Western blotting. Validate using appropriate positive controls, such as human embryonic or neuronal cell lines.
• For ELISA, the antibody can be used in indirect detection assays; however, users should optimize antigen coating and antibody dilutions for their specific experimental setup.
• Because NAT9 is expressed in embryonic brain, consider using developmental tissue samples or differentiated neuronal cultures when assessing endogenous expression levels.
• Cross-reactivity with other species has not been tested; therefore, we recommend verifying reactivity if using non-human samples.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we streamline procurement for diagnostic manufacturers and research labs by offering a comprehensive portfolio of validated IVD raw materials. The NAT9 rabbit polyclonal antibody supports investigations into tubulin acetylation and neurobiology, bridging basic research and assay development. Reach out to leverage our one-stop sourcing capabilities, including bulk ancillary reagents and customized raw-material solutions.