Anti-NAT8B Rabbit Polyclonal Antibody (CML2, ATase1) for WB, IHC-P, ELISA - Q9UHF3

Core Product Specifications and Parameters

Biological Background: NAT8B Function and Localization

• NAT8B (N-acetyltransferase 8B), also known as acetyltransferase 1 (ATase1), camello-like protein 2 (CML2), or Hcml2, belongs to the camello family of N-acetyltransferases.
• It functions as an endoplasmic reticulum (ER) membrane-bound lysine N6-acetyltransferase that catalyzes the acetyl-CoA–dependent acetylation of lysine residues on substrate proteins within the ER lumen, modulating protein stability and function.
• NAT8B acetylates the stem cell marker PROM1 (CD133), regulating its expression and potentially influencing apoptosis. Related references: PMID:24556617
• It also acetylates and stabilizes the immature form of BACE1 (beta-secretase 1), increasing its steady-state levels in neurons and thereby affecting amyloid beta-peptide formation, a key process in Alzheimer’s disease pathology. Related references: PMID:19011241 / PMID:22267734
• Subcellularly, NAT8B localizes to the ER membrane and the ER-Golgi intermediate compartment (ERGIC) membrane, consistent with its role in early secretory pathway protein modifications.
• UniProt annotations highlight its involvement in acetylation, acyltransferase activity, and its transmembrane helix topology, with a calculated molecular weight of approximately 25 kDa (25366 Da).

Experimental Guidance and Technical Tips

• The immunogen corresponds to a central region (amino acids 75–165) of human NAT8B, which is well conserved across human, mouse, and rat, supporting the reported species cross-reactivity.
• For Western blot (WB), consider using a total protein load of 20–50 µg lysate and validate detection of a band migrating near 25 kDa; reducing conditions are recommended.
• In immunohistochemistry (IHC-P), antigen retrieval conditions (e.g., citrate buffer pH 6.0) and tissue type should be optimized, as NAT8B expression levels vary.
• For ELISA applications, the antibody can be used as either capture or detection reagent, but pairing with a matched antibody pair should be tested for optimal signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs by providing validated raw materials such as this anti-NAT8B polyclonal antibody, along with optimized monoclonal/polyclonal pairs, bulk ancillary reagents, and custom sourcing for rare targets. Whether your work focuses on protein acetylation, ER stress pathways, or neurodegenerative disease markers, CamelBio streamlines procurement with consistent quality and technical consultation to help move your assays from concept to clinic.