Anti-NAT8 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9UHE5

Core Product Specifications and Parameters

Biological Background: NAT8 Function and Localization

• NAT8 (N-acetyltransferase 8), also known as ATase2, Camello-like protein 1, CCNAT, or GLA, is a member of the GNAT family of N-acetyltransferases, with a predicted molecular weight of approximately 26 kDa.
• As an endoplasmic reticulum (ER)-membrane-bound enzyme, it catalyzes N6-acetylation of lysine residues in ER lumenal proteins such as PROM1 and BACE1, utilizing acetyl-CoA as the acetyl donor. Related references: PMID:19011241, PMID:22267734, PMID:24556617
• This acetylation activity may regulate apoptosis through modulation of PROM1 expression and influence amyloid beta-peptide secretion via BACE1 acetylation in neuronal cells. Related references: PMID:19011241, PMID:24556617
• NAT8 is also involved in maintaining ER protein homeostasis and regulating reticulophagy.
• Alternatively, NAT8 functions in phase II detoxification by acetylating the free alpha-amino group of cysteine S-conjugates to form mercapturic acids, the final step in glutathione-mediated elimination of reactive electrophiles. Related references: PMID:20392701
• At the subcellular level, NAT8 localizes to the endoplasmic reticulum-Golgi intermediate compartment and ER membranes.
• NAT8 is preferentially expressed in liver and kidney, with detectable protein levels also observed in brain.
• Key associated annotations include acyltransferase, transferase, transmembrane, and signal-anchor features.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody corresponds to amino acids 61–220 of human NAT8, a region that may be conserved across species, consistent with the observed cross-reactivity with mouse and rat.
• For Western blot (WB) applications, a band at approximately 26 kDa is expected. Validate detection in relevant tissue lysates such as liver or kidney, where NAT8 is highly expressed.
• Immunohistochemistry (IHC-P) may require antigen retrieval optimization; consider testing on paraffin-embedded liver or kidney sections.
• ELISA performance should be evaluated under the specific assay conditions; this antibody can serve as a capture or detection reagent, but appropriate pairing and titration are recommended.
• As a polyclonal antibody, lot-to-lot consistency may vary; it is advisable to include appropriate controls in each experiment.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw material partner, CamelBio supports diagnostic manufacturers and research laboratories with reliable access to high-quality antibodies and ancillary reagents. This anti-NAT8 polyclonal antibody is well-suited for research on protein acetylation, ER biology, and detoxification pathways, and can be integrated into assay development workflows. Beyond individual products, CamelBio offers validated antibody pairs, bulk monoclonal and polyclonal antibody supplies, and customized sourcing for rare targets, bridging the gap from concept to clinical application.