Anti-NAT10 Rabbit Monoclonal Antibody [KD Validated] for WB, IP, IF/ICC, IHC-P, ELISA - Q9H0A0

Core Product Specifications and Parameters

Biological Background: NAT10 Function and Localization

• NAT10 is an RNA cytidine acetyltransferase that catalyzes the formation of N4-acetylcytidine (ac4C) on mRNAs, 18S rRNA, and tRNAs, enhancing mRNA stability and translation. Related references: PMID:30449621, PMID:35679869
• NAT10 catalyzes ac4C modification at position 1842 in 18S rRNA (and possibly position 1337), which is essential for early nucleolar cleavages of precursor rRNA during 18S rRNA synthesis. Related references: PMID:25411247, PMID:25653167
• It is a component of the small subunit (SSU) processome, the first precursor of the small eukaryotic ribosomal subunit, and is involved in ribosome biogenesis. Related references: PMID:34516797
• NAT10 also acetylates lysine residues of proteins such as histones, microtubules, p53/TP53, and MDM2 in vitro, though the in vivo relevance is uncertain. Related references: PMID:14592445, PMID:17631499, PMID:19303003
• It activates telomerase activity by stimulating TERT transcription and may regulate telomerase subunit assembly, disassembly, and localization. Related references: PMID:14592445, PMID:18082603
• NAT10 is involved in regulating centrosome duplication by acetylating CENATAC during mitosis, promoting SASS6 proteasomal degradation. Related references: PMID:31722219
• Subcellular localization: primarily in the nucleolus and nucleus, also found at the midbody.
• Protein aliases include ALP, Kre33, NET43; belongs to the acetyltransferase family and features ATP-binding and nucleotide-binding domains.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (aa 676-1025) of human NAT10; consider epitope accessibility in different applications.
• For WB, detected bands around 116 kDa and 107 kDa; possible isoform-specific detection; validate loading controls and blocking conditions in your sample system.
• This antibody is reported to work in multiple applications; for IP, confirm antibody compatibility with native protein conformations and lysis buffer conditions.
• Cross-reactivity with mouse and rat suggests suitability for animal models; however, verify antibody specificity in the relevant tissue or cell line.
• For IF/ICC and IHC-P, optimize fixation and permeabilization protocols to preserve epitope recognition and minimize background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting from concept to clinic. This NAT10 antibody supports investigations into RNA modification and ribosome biogenesis. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing to streamline your assay development.