Anti-NAT10 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q9H0A0

Core Product Specifications and Parameters

Biological Background: NAT10 Function and Localization

• NAT10 is an RNA cytidine acetyltransferase that catalyzes the formation of N4-acetylcytidine (ac4C) on mRNAs, 18S rRNA, and tRNAs, playing a key role in epitranscriptomic regulation. Related references: PMID:25411247 PMID:25653167 PMID:30449621
• The ac4C modification of mRNAs by NAT10 enhances mRNA stability and translation efficiency, frequently occurring at wobble cytidine sites. Related references: PMID:30449621 PMID:35679869
• NAT10 is essential for ribosome biogenesis, mediating early nucleolar cleavages of precursor rRNA and acting as a component of the small subunit processome. Related references: PMID:25411247 PMID:25653167 PMID:34516797
• The protein localizes predominantly to the nucleus and nucleolus, and is also detected at the midbody during cell division.
• Besides RNA acetylation, NAT10 can acetylate protein substrates such as histones, p53, and microtubules in vitro, though its in vivo protein acetyltransferase activity remains uncertain. It also activates telomerase and regulates centrosome duplication. Related references: PMID:14592445 PMID:17631499 PMID:19303003 PMID:18082603 PMID:31722219
• NAT10 is subject to alternative splicing and post-translational modifications including acetylation and phosphorylation, and contains ATP-binding and nucleotide-binding domains.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 750-1025), ensuring detection of full-length NAT10. Verify reactivity in your sample system, especially if truncated isoforms are suspected.
• For Western blotting, expect a band around 116 kDa. Use appropriate positive controls such as HeLa or HEK293 cell lysates, which are known to express NAT10.
• In immunohistochemistry (IHC-P), optimize antigen retrieval conditions (e.g., citrate buffer pH 6.0 or EDTA pH 8.0) and validate staining patterns against known nuclear/nucleolar localization.
• For ELISA applications, pair with a suitable detection antibody and include proper negative and positive controls to assess specificity.

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CamelBio supports diagnostic manufacturers and research labs with a comprehensive portfolio of IVD raw materials, including validated antibodies like this anti-NAT10 clone for epitranscriptomics and cancer research. We offer monoclonal/polyclonal antibodies, bulk ancillary reagents, and specialized sourcing for rare targets—helping you move from discovery to clinical application efficiently.