Anti-NAT1 Rabbit Polyclonal Antibody for WB and ELISA - P18440

Core Product Specifications and Parameters

Biological Background: NAT1 Function and Localization

• NAT1 (arylamine N-acetyltransferase 1), also designated AAC1, MNAT, NATI, or NAT-1, is a member of the phase II xenobiotic-metabolizing enzyme family that catalyzes acetyl group transfer from acetyl-CoA to arylamine substrates.
• It plays a central role in the detoxification of numerous hydrazine and arylamine drugs, reducing their pharmacological activity and facilitating excretion.
• Conversely, NAT1 can bioactivate certain environmental procarcinogens, including heterocyclic amines, through O-acetylation of N-hydroxylated metabolites, generating electrophilic intermediates capable of binding DNA.
• The enzyme is predominantly localized in the cytoplasm and exhibits ubiquitous tissue distribution, reflecting its broad role in cellular protection and chemical processing.
• Human NAT1 has a molecular mass of approximately 34 kDa and is subject to acetylation-based regulatory mechanisms.
• Genetic variants of NAT1 are associated with differential acetylation capacity, impacting individual susceptibility to drug-induced toxicity and carcinogenesis.

Experimental Guidance and Technical Tips

• The immunogen encompasses nearly the full-length human NAT1 (residues 1–290), which may confer reactivity to multiple allelic forms; however, variant-specific detection should be confirmed with recombinant proteins.
• For Western blotting, consider a starting dilution range of 1:500–1:2000 using whole-cell lysates from hepatocyte-derived cell lines (e.g., HepG2) or human liver tissue, where a band at ~34 kDa is expected.
• In ELISA applications, the antibody can be employed as a capture or detection reagent; optimizing coating concentrations and pairing with a monoclonal antibody may improve assay specificity.
• Cross-reactivity has been validated against human NAT1; performance in other species is not guaranteed and should be individually tested.
• Upon arrival, store aliquots at -20°C in a non-frost‑free freezer to minimize freeze‑thaw degradation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines the procurement of IVD raw materials for drug metabolism and toxicology researchers by offering a single point of access to antibodies, antigens, and ancillary reagents. This anti-NAT1 polyclonal antibody is part of a broader portfolio that includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to support assay development and manufacturing. Whether you need standard catalog items or rare-target sourcing, our team facilitates a reliable supply chain from concept to clinic.