Anti-NAT1 Rabbit Monoclonal Antibody for IF/ICC, ELISA - P18440

Core Product Specifications and Parameters

Biological Background: NAT1 Function and Localization

• NAT1 (Arylamine N-acetyltransferase 1) is a phase II xenobiotic-metabolizing enzyme that acetylates aromatic amines and hydrazines, playing a critical role in drug detoxification.
• It catalyzes both N-acetylation (detoxification) and O-acetylation (bioactivation) of substrates, thereby capable of activating certain procarcinogens.
• NAT1 is a monomorphic enzyme expressed in various tissues, unlike its polymorphic counterpart NAT2; it is also known as AAC1, MNAT, NAT-1, and NATI.
• The protein localizes to the cytoplasm, where it exerts its acetyltransferase activity, as annotated by the keywords "Cytoplasm" and "Transferase".
• Acetylation is a key post-translational modification that may regulate NAT1 function and stability.
• The theoretical molecular weight of human NAT1 is approximately 33.9 kDa, with an observed band often detected around 34 kDa in Western blotting (although this antibody is not validated for WB, the protein weight matches the product specification).

Experimental Guidance and Technical Tips

• The immunogen targets the N-terminal region (amino acids 1-100) of human NAT1; validate cross-reactivity with mouse and rat orthologs experimentally, as sequence homology may vary.
• For immunofluorescence/immunocytochemistry (IF/ICC), permeabilize cells with 0.1–0.2% Triton X-100 or saponin to allow antibody access to cytoplasmic NAT1.
• In ELISA, consider pairing this monoclonal antibody with a detection antibody specific for a distinct NAT1 epitope, or use it as a capture antibody; optimize coating conditions and concentrations.
• Include appropriate controls: human liver cell lines (e.g., HepG2) as positive control and NAT1-knockout or siRNA-treated cells as negative control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw material partner for diagnostic manufacturers and research laboratories, offering reliable antibodies for drug metabolism and cancer biomarkers. For NAT1-related projects, we supply this validated rabbit monoclonal antibody alongside additional reagents such as secondary antibodies, ELISA substrates, and blocking buffers. Contact us for bulk orders, custom antibody development, and technical consulting to accelerate your assay development.