Anti-NASP Polyclonal Antibody for WB, IHC-P, ELISA - P49321

Core Product Specifications and Parameters

Biological Background: NASP Function and Localization

• NASP (Nuclear Autoantigenic Sperm Protein) is a histone chaperone that binds and stabilizes soluble histone H3-H4 complexes, maintaining a reserve pool not bound to chromatin (PubMed:22195965). Related references: PMID:22195965
• NASP plays a critical role in DNA replication, cell cycle progression, and cell proliferation by ensuring proper histone supply and chromatin assembly.
• It forms a cytoplasmic complex with HSP90 and H1 linker histones, stimulating HSP90 ATPase activity, and facilitating the transport of H1 histones to the nucleus.
• Two main isoforms exist: isoform 1 is testis- and sperm-specific, while a shorter somatic isoform (lacking the testis-specific region) is expressed in other tissues.
• Subcellular localization is both cytoplasmic and nuclear, reflecting its shuttling role in histone transport and assembly.
• The protein contains TPR (tetratricopeptide repeat) domains and a coiled-coil region, which mediate interactions with histones and chaperones.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination (Ubl conjugation), as indicated by proteomics data.

Experimental Guidance and Technical Tips

• For Western blotting, the antibody is expected to detect a band around 85 kDa, consistent with the predicted molecular weight of the target isoform (NP_689511.2). Verify band specificity using appropriate controls, especially given the polyclonal nature.
• For immunohistochemistry (IHC-P), consider optimizing antigen retrieval conditions to unmask the epitope, as nuclear proteins may require stringent protocols.
• In ELISA applications, the recombinant protein immunogen region (aa180-449) suggests the antibody can detect both somatic and testis-specific isoforms, but validate in the relevant sample matrix.
• Since the antibody cross-reacts with mouse and rat, it may be suitable for comparative studies; however, confirm reactivity in your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized polyclonal antibodies like this anti-NASP antibody, and bulk ancillary reagents. For chromatin and cell cycle research, CamelBio ensures seamless procurement of high-quality antibodies and rare-target raw materials to accelerate your diagnostic development from concept to clinic.