Anti-NAPSA Polyclonal Antibody for WB, ELISA - O96009

Core Product Specifications and Parameters

Biological Background: Napsin-A Function and Localization

• Napsin-A (aspartyl protease 4, ASP4, napsin-1) is an aspartyl protease encoded by the NAPSA gene.
• It may be involved in the processing of pneumocyte surfactant precursors.
• Expressed predominantly in adult lung (type II pneumocytes) and kidney, with lower levels in fetal lung, adult spleen, and peripheral blood leukocytes.
• Subcellular localization: secreted; contains a signal peptide and exists as a zymogen.
• Classified as an aspartyl protease, hydrolase; features disulfide bonds and glycosylation.
• Post‑translational modifications include direct protein sequencing and proteomic identification.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 64–190) corresponds to a region within the mature napsin‑A protein, likely contributing to specificity for the processed form.
• For Western blot, expect a band at approximately 45 kDa; consider lysates from human lung or kidney tissue as positive controls.
• Cross‑reactivity with rat napsin‑A has been observed; validate in rat lung or kidney samples using appropriate loading controls.
• ELISA applications may benefit from coating recombinant napsin‑A or native protein in tissue homogenates; optimize detection conditions empirically.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio is your one‑stop partner for IVD raw materials, offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare‑target sourcing. This NAPSA polyclonal antibody supports surfactant‑related research and diagnostic development, and we can assist with complementary reagents and custom supply solutions to streamline your project from concept to clinic.