Anti-NAPRT Polyclonal Antibody for WB, ELISA - Q6XQN6

Core Product Specifications and Parameters

Biological Background: NAPRT Function and Localization

• NAPRT, encoded by the NAPRT gene, is also designated as Nicotinate phosphoribosyltransferase, NAPRT1, PP3856, and FHA-HIT-interacting protein.
• NAPRT catalyzes the ATP-dependent formation of beta-nicotinate D-ribonucleotide from nicotinate and 5-phospho-D-ribose 1-phosphate, the initiating step of NAD biosynthesis from nicotinic acid. Related references: PMID:17604275 | PMID:21742010 | PMID:26042198
• This enzymatic activity is crucial for preventing cellular oxidative stress through maintenance of NAD levels. Related references: PMID:17604275
• Subcellularly, NAPRT is predominantly localized to the cytoplasm and cytosol.
• The protein is a member of the nicotinate phosphoribosyltransferase family, requiring magnesium or manganese as cofactors for its transferase and ligase functions.
• Post-translational modifications include phosphorylation, and alternative splicing generates multiple isoforms.
• The full-length human NAPRT has a predicted molecular weight of approximately 57.6 kDa (57578 Da), consistent with the observed band at 58 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 309-538); the antibody may recognize both full-length and C-terminal fragments—validate in the relevant sample system.
• For Western blot, a band near 58 kDa is expected; consider using reducing conditions and appropriate blocking reagents.
• In ELISA, titrate the antibody against the target protein to determine the optimal working dilution for your assay.
• Cross-reactivity with mouse and rat NAPRT has been indicated; confirm sequence homology and test in your specific experimental models.
• Since the antibody is raised in rabbit, use an anti-rabbit secondary antibody for detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material partner, offering a streamlined procurement pathway for diagnostic developers and research labs. For studies focusing on NAD metabolism and oxidative stress, we can supply validated antibody pairs, optimized polyclonal antibodies like this anti-NAPRT product, bulk ancillary reagents, and custom sourcing for rare-target raw materials. Reach out to explore how we can support your project from concept to clinic.