Anti-NAPG Rabbit Polyclonal Antibody for WB and ELISA - Q99747

Core Product Specifications and Parameters

Biological Background: NAPG Function and Localization

• NAPG, also known as gamma-soluble NSF attachment protein or GAMMASNAP, encoded by the NAPG (SNAPG) gene, is a member of the soluble NSF attachment protein (SNAP) family.
• It plays a critical role in intracellular membrane trafficking by functioning in vesicular transport between the endoplasmic reticulum and the Golgi apparatus.
• The protein is localized to the membrane of the Golgi apparatus, consistent with its role in ER-Golgi transport.
• NAPG is subject to alternative splicing, yielding multiple isoforms that may have distinct functional properties.
• It is a phosphoprotein, suggesting regulation by phosphorylation in transport processes.
• The protein is widely expressed and has been identified in proteomics analyses, indicating its ubiquitous role in cellular transport.

Experimental Guidance and Technical Tips

• For Western blot applications, consider using whole-cell lysates from human, mouse, or rat cell lines and validate detection of a band at approximately 35 kDa.
• The immunogen corresponds to the N-terminal region (amino acids 1-312), which may be suitable for detecting both full-length and alternatively spliced isoforms.
• In ELISA applications, optimize coating antigen concentration and antibody dilution using the recombinant immunogen or purified protein to establish assay conditions.
• Verify cross-reactivity in mouse and rat tissues by comparing band patterns, as sequence conservation in this region supports multi-species recognition.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with comprehensive access to IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This anti-NAPG antibody supports intracellular trafficking and vesicular transport research, areas critical for understanding cellular logistics and disease mechanisms. Leveraging our sourcing network, we can efficiently supply rare-target raw materials to streamline your development from concept to clinic.