Anti-NAPG (GAMMASNAP) Rabbit Polyclonal Antibody for WB and ELISA - Q99747

Core Product Specifications and Parameters

Biological Background: NAPG Function and Localization

• NAPG encodes gamma-soluble NSF attachment protein (gamma-SNAP), a key component of the SNARE complex involved in intracellular membrane fusion.
• Gamma-SNAP is required for vesicular transport between the endoplasmic reticulum and the Golgi apparatus, facilitating the docking and fusion of transport vesicles.
• The protein localizes predominantly to the membrane of the Golgi apparatus, where it participates in the regulation of ER-Golgi trafficking.
• NAPG is a member of the SNAP family, which includes alpha, beta, and gamma isoforms, each with distinct but partially overlapping functions in membrane traffic.
• Post-translational modifications include phosphorylation, which may modulate its activity in membrane trafficking pathways.
• Alternative splicing generates multiple transcript variants, potentially contributing to functional diversity.
• Keywords: ER-Golgi transport, Golgi apparatus, membrane, phosphoprotein, protein transport, and reference proteome.

Experimental Guidance and Technical Tips

• The immunogen comprises the full-length protein (aa 1-312), which may confer high specificity for endogenous NAPG detection. Consider epitope conservation when designing blocking peptides or confirming antibody specificity in knockout models.
• For Western blotting, a band at approximately 35 kDa is expected; optimize lysate preparation and antibody dilution using appropriate positive controls (e.g., HeLa, HEK293) and validate in relevant sample systems.
• For ELISA, consider coating antigen and detection conditions based on the recombinant immunogen; include negative controls to assess background binding.
• Cross-reactivity with mouse and rat orthologs is reported; verify reactivity in your experimental species by sequence alignment and pilot experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research teams with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. For projects centered on membrane trafficking and protein transport, we offer one-stop access to NAPG-related tools and can assist in sourcing rare-target raw materials or developing custom assay components. From concept to clinic, CamelBio bridges procurement gaps with reliable B2B supply solutions.