Anti-NAP1L1 Monoclonal Antibody for WB, IF/ICC, ELISA - P55209

Core Product Specifications and Parameters

Biological Background: NAP1L1 Function and Localization

• NAP1L1 is a histone chaperone that facilitates the nuclear import of H2A-H2B and nucleosome assembly. Related references: PMID:20002496 PMID:21211722 PMID:26841755
• NAP1L1 participates in transcription-coupled nucleotide excision repair (TC-NER) by enhancing ERCC6-mediated chromatin remodeling. Related references: PMID:28369616
• It stimulates homologous recombination (HR) DNA double-strand break repair via RAD51 and RAD54. Related references: PMID:24798879
• Plays a key role in embryonic neurogenesis by promoting neural progenitor proliferation and inhibiting neuronal differentiation.
• Ubiquitously expressed and localizes to the nucleus, chromosomes, melanosomes, and cytoplasm.
• Undergoes multiple post-translational modifications including acetylation, phosphorylation, and prenylation.
• Also implicated in viral reactivation, positively regulating Epstein-Barr virus reactivation and assisting human herpesvirus 8 nucleosome assembly.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminus (aa 1-100); consider using this region as a positive control in peptide blocking assays.
• For Western blotting, a band around 45 kDa is expected; pre-stained molecular weight markers are recommended for accurate size determination.
• In immunofluorescence/immunocytochemistry, nuclear localization should be confirmed; permeabilization conditions may need optimization.
• The antibody cross-reacts with mouse and rat, making it suitable for studies in these model organisms—validate detection in your tissue or cell line of interest.
• ELISA performance may depend on coating antigen; consider using recombinant NAP1L1 or peptide for direct assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials, including validated antibody pairs and optimized monoclonal antibodies like this Anti-NAP1L1 Rabbit mAb. With expertise in chromatin biology and DNA repair targets, we support your development needs—from bulk ancillary reagents to rare target sourcing—helping you move efficiently from concept to clinic.