Anti-NANS Rabbit Polyclonal Antibody for WB, ELISA - Q9NR45

Core Product Specifications and Parameters

Biological Background: NANS Function and Localization

• Encoded by the NANS gene (also known as SAS), N-acetylneuraminate-9-phosphate synthase is a key enzyme in sialic acid metabolism, with the alias Sialic acid synthase reflecting its role.
• Catalyzes the condensation of phosphoenolpyruvate (PEP) and N-acetylmannosamine 6-phosphate (ManNAc-6-P) to synthesize N-acetylneuraminate-9-phosphate (Neu5Ac-9-P), the activated precursor of N-acetylneuraminic acid (sialic acid).
  Related references: PMID:10749855
• Also utilizes PEP and D-mannose 6-phosphate (Man-6-P) to produce 3-deoxy-D-glycero-beta-D-galacto-non-2-ulopyranosonate 9-phosphate (KDN-9-P), the precursor of deaminoneuraminic acid.
  Related references: PMID:10749855
• Essential for normal brain and skeletal development; mutations in NANS are linked to intellectual disability and dwarfism.
  Related references: PMID:27213289
• Ubiquitously expressed across tissues, consistent with the housekeeping role of sialic acid production.
• Post-translational modifications include acetylation and phosphorylation, and the protein has been structurally characterized at high resolution.
• UniProt annotations highlight it as a transferase, with direct protein sequencing and proteomics identification supporting its reference proteome status.
• The calculated molecular mass is 40.3 kDa, and the antibody detects a band at approximately 40 kDa in western blotting, aligning with the product specification.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 260–359, a C-terminal region, which may confer specificity for NANS over related synthases; verify in the target sample matrix.
• For western blotting, expect a predominant band around 40 kDa; use knockout‑validated lysates as a negative control to confirm antibody specificity.
• In ELISA, consider using the recombinant immunogen or purified recombinant NANS as a coating antigen and titrate the antibody for optimal signal-to-noise ratio.
• Given its knockout validation, this antibody is particularly suited for experiments requiring unambiguous target identification, such as CRISPR knockdown or siRNA studies.
• Reactivity with species other than human has not been characterized; if cross‑species applications are needed, validate the antibody in the relevant cell or tissue extracts.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a streamlined, one‑stop channel for IVD raw‑material procurement. This anti‑NANS polyclonal antibody exemplifies our capability to supply validated antibodies for sialic acid metabolism and developmental biology research. Beyond individual reagents, we provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and dedicated sourcing support for rare targets to accelerate your project from concept to clinic.