Anti-NANP Rabbit Polyclonal Antibody for WB and ELISA - Q8TBE9

Core Product Specifications and Parameters

Biological Background: NANP Function and Localization

• NANP (C20orf147) encodes N-acylneuraminate-9-phosphatase, also referred to as HDHD4 (Haloacid dehalogenase-like hydrolase domain-containing protein 4) and Neu5Ac-9-Pase.
• It is a member of the haloacid dehalogenase (HAD) superfamily of hydrolases, bearing a characteristic haloacid dehalogenase-like hydrolase domain.
• The enzyme catalyzes the dephosphorylation of N-acetylneuraminate 9-phosphate (Neu5Ac-9-P) to generate N-acetylneuraminic acid (sialic acid), a key step in sialic acid biosynthesis. Related references: PMID:14235556 | PMID:16237198 | PMID:23747226
• It can also recognize and dephosphorylate N-glycoloylneuraminate 9-phosphate as a substrate. Related references: PMID:14235556
• Functional studies and keyword annotations indicate a magnesium-dependent catalytic mechanism.
• The predicted molecular weight from the primary sequence is ~27.8 kDa, which aligns with the experimentally observed band at 28 kDa in immunoblots.

Experimental Guidance and Technical Tips

• The immunogen consists of recombinant full-length human NANP (aa 1-248), suggesting the polyclonal antibody targets multiple epitopes across the entire protein.
• For Western blot, consider initial screening at dilutions between 1:500 and 1:2,000, using whole-cell lysates from human, mouse, or rat tissues. Confirm specificity by pre-incubating the antibody with the immunogen.
• In ELISA, the polyclonal nature may provide robust signal; if cross-reactivity with other HAD family members is suspected, protein‑A purification or cross‑adsorption steps can help reduce background.
• Store the antibody at −20 °C in small working aliquots to avoid freeze‑thaw cycles; for long‑term storage, consider −80 °C as recommended for polyclonal antibodies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic developers with a broad portfolio of IVD raw materials, including validated polyclonal antibodies such as this anti-NANP antibody. Whether you need antibody pairs, bulk ancillary reagents, or custom sourcing for rare targets in sialic acid metabolism, we streamline supply from concept to clinic. Contact our team to discuss how we can support your next assay development project.