Anti-Nanog Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9H9S0

Core Product Specifications and Parameters

Biological Background: Nanog Function and Localization

• Homeobox protein NANOG is a transcription regulator critical for inner cell mass and embryonic stem (ES) cell proliferation and self-renewal.
• Imposes pluripotency on ES cells and prevents differentiation towards extraembryonic endoderm and trophectoderm lineages.
• Acts as both a transcriptional activator and repressor; binds to DNA consensus sequences 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3', and to the POU5F1/OCT4 promoter. Related references: PMID:25825768
• Blocks bone morphogenetic protein-induced mesoderm differentiation by physically interacting with SMAD1 and interfering with coactivator recruitment.
• Able to autorepress its own expression in differentiating ES cells via interaction with ZNF281/ZFP281, leading to NuRD complex recruitment and repression.
• Subcellular localization is exclusively nuclear, consistent with its role as a transcription factor.
• Expressed in testicular carcinoma and derived germ cell tumors, fetal gonads, and embryonic carcinoma cell lines; not expressed in many somatic organs.
• Keywords: alternative splicing, homeobox domain, developmental protein, DNA-binding, nuclear protein.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1–105), which may detect full-length and N-terminal isoforms; verify specificity in the relevant sample system.
• For WB, consider a predicted molecular weight of ~35 kDa (observed 35 kDa per supplier), but actual migration may vary; use appropriate positive and negative controls.
• For IHC-P, validate in tissue sections with known NANOG expression, such as embryonic carcinoma or testicular germ cell tumors; optimize antigen retrieval conditions.
• ELISA performance depends on coating strategy and detection format; consider using recombinant NANOG protein standards for assay calibration.
• As a polyclonal antibody, lot-to-lot consistency should be verified; evaluate each new lot in your specific assay context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research teams with validated raw materials including anti-NANOG antibodies for stem cell pluripotency, developmental biology, and cancer studies. We offer bulk polyclonal antibody lots, optimized monoclonal/polyclonal antibody pairs, and ancillary reagents to streamline your supply chain. From rare-target sourcing to technical consulting, rely on CamelBio for end-to-end IVD raw-material support—advancing your projects from concept to clinic.